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Submit ReviewMag-Fura-2 AM is a cell-permeable fluorescent magnesium ion (Mg2+) indicator. It shows selectivity for Mg2+ over other bivalent ions (Zn2+, Cu2+ and Ca2+). It exhibits >13,000-fold selectivity for Mg2+ ions over Ca2+ ions (Mg2+ Kd = 1.9 nM vs Ca2+ Kd = 25 μM). Excitation and emission maxima (λ) are 369 and 511 nm, respectively. Mag-Fura-2 AM can be used as an indirect indicator of ATP consumption/production.
It is recommended to prepare stock solutions in DMSO.
λabs | 369 nm |
---|---|
λem | 511 nm |
Extinction Coefficient (ε) | 31000 M-1cm-1 |
Cell Permeable | Yes |
Application | Confocal Imaging, Flow Cytometry |
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral ViewerM. Wt | 722.57 |
Formula | C30H30N2O19 |
Storage | Store at -20°C |
Purity | ≥95% (HPLC) |
CAS Number | 130100-20-8 |
PubChem ID | 131176 |
InChI Key | PCDUVTXECKTHBH-UHFFFAOYSA-N |
Smiles | O=C(C1=CN=C(C2=CC3=C(C=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCC(OCOC(C)=O)=O)=C3)O2)O1)OCOC(C)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
References are publications that support the biological activity of the product.
Schütz et al (2021) Trophectoderm cell failure leads to peri-implantation lethality in Trpm7-deficient mouse embryos. Cell Rep. 37 109851 PMID: 34686339
Watanabe et al (2022) Chronic magnesium deficiency causes reversible mitochondrial permeability transition pore opening and impairs hypoxia tolerance in the rat heart. J.Pharmacol.Sci. 148 238 PMID: 35063139
Cheng et al (2021) Variability of mitochondrial energy balance across brain regions. J.Neurochem. 157 1234 PMID: 33190229
If you know of a relevant reference for Mag-Fura-2 AM, please let us know.
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