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MD13

Cat. No. 7503 Submit a Review Datasheet / COA / SDS
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Description: Potent MIF Degrader (PROTAC®)
Chemical Name: 8-((2-(2,6-Dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)amino)-N-(4-(7-hydroxy-2-oxo-2H-benzo[e][1,3]oxazin-3(4H)-yl)phenyl)octanamide
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews
Literature (2)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for MD13

MD13 is a potent macrophage migration inhibitory factor (MIF) Degrader (PROTAC®; DC50 < 100 nM; Dmax = 92% after 6 h). MD13 comprises the cereblon ligand Pomalidomide (Cat. No. 6302) joined by a linker to a MIF inhibitor. MD13 reduces MIF levels, arrests cells at the G2/M phase and inhibits proliferation of A549 lung carcinoma cells. Growth of A549 cell 3D spheroids is also inhibited by MD13.

Negative control MD15 (Cat. No. 7504) and MIF antibodies validated for Simple Western™ (automated Western) instruments and Western Blot also available: Catalog # AF-289-PB and MAB289.

PROTAC® is a registered trademark of Arvinas Operations, Inc., and is used under license.

Licensing Information

Sold with kind permission of Professor Dekker.

Technical Data for MD13

M. Wt 653.69
Formula C35H35N5O8
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 2758431-97-7
InChI Key HAHDZDUOFHMMEA-UHFFFAOYSA-N
Smiles O=C1N(CC2=CC=C(C=C2O1)O)C3=CC=C(C=C3)NC(CCCCCCCNC4=C(C5=CC=C4)C(N(C5=O)C6CCC(NC6=O)=O)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for MD13

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 65.37 100

Preparing Stock Solutions for MD13

The following data is based on the product molecular weight 653.69. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.53 mL 7.65 mL 15.3 mL
5 mM 0.31 mL 1.53 mL 3.06 mL
10 mM 0.15 mL 0.76 mL 1.53 mL
50 mM 0.03 mL 0.15 mL 0.31 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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References for MD13

References are publications that support the biological activity of the product.

Xiao et al (2021) Proteolysis targeting chimera (PROTAC) for macrophage migration inhibitory factor (MIF) has anti-proliferative activity in lung cancer cells. Angew.Chem.Int.Ed.Engl. 60 17514 PMID: 34018657

If you know of a relevant reference for MD13, please let us know.

View Related Products by Target

Keywords: MD13, MD13 supplier, potent, MIF, Degraders, degrades, macrophage, migration, inhibitory, factor, targeted, protein, degradation, TPD, PROTAC, Cytokines, Other, 7503, Tocris Bioscience

Citations for MD13

Citations are publications that use Tocris products.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

TPD and Induced Proximity Research Product Guide

TPD and Induced Proximity Research Product Guide

This brochure highlights the tools and services available from Bio-Techne to support your Targeted Protein Degradation and Induced Proximity research, including:

  • Active Degraders
  • TAG Degradation Platform
  • Degrader Building Blocks
  • Assays for Protein Degradation
  • Induced Proximity Tools
Targeted Protein Degradation Poster

Targeted Protein Degradation Poster

Degraders (e.g. PROTACs) are bifunctional small molecules, that harness the Ubiquitin Proteasome System (UPS) to selectively degrade target proteins within cells. They consist of three covalently linked components: an E3 ubiquitin ligase ligand, a linker and a ligand for the target protein of interest. Authored in-house, this poster outlines the generation of a toolbox of building blocks for the development of Degraders. The characteristics and selection of each of these components are discussed. Presented at EFMC 2018, Ljubljana, Slovenia