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ML 210

Cat. No. 6429 1 Citation Submit a Review Datasheet / COA / SDS
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Description: Selectively kills mutant HRAS-expressing cells; glutathione peroxidase inhibitor; induces ferroptosis
Chemical Name: [4-[Bis(4-chlorophenyl)methyl]-1-piperazinyl](5-methyl-4-nitro-3-isoxazolyl)methanone
Purity: ≥98% (HPLC)
Datasheet
Citations (1)
Reviews
Literature (4)
Pathways (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for ML 210

ML 210 is a glutathione peroxidase (GPX4) inhibitor. Kills mutant RAS-expressing cell lines (IC50 values are 71 and 272 nM for HRAS G12v mutant expressing cell lines BJeLR and BJeH-LT, respectively). Exhibits 4-fold selectivity for HRAS mutant-expressing cell lines. Induces ferroptosis in tumor initiation "persister" cells and drug-resistant tumor cells.

Compound Libraries for ML 210

ML 210 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for ML 210

M. Wt 475.32
Formula C22H20Cl2N4O4
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1360705-96-9
PubChem ID 49766530
InChI Key VIBHJPDPEVVDTB-UHFFFAOYSA-N
Smiles CC1=C([N+]([O-])=O)C(C(N2CCN(C(C3=CC=C(C=C3)Cl)C4=CC=C(C=C4)Cl)CC2)=O)=NO1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for ML 210

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 23.77 50

Preparing Stock Solutions for ML 210

The following data is based on the product molecular weight 475.32. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 4.21 mL 21.04 mL 42.08 mL
2.5 mM 0.84 mL 4.21 mL 8.42 mL
5 mM 0.42 mL 2.1 mL 4.21 mL
25 mM 0.08 mL 0.42 mL 0.84 mL

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Product Datasheets for ML 210

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References for ML 210

References are publications that support the biological activity of the product.

Weïwer et al (2012) Development of small-molecule probes that selectively kill cells induced to express mutant RAS. Bioorg.Med.Chem.Lett. 22 1822 PMID: 22297109

Yang et al (2014) Regulation of ferroptotic cancer cell death by GPX4. Cell 156 317 PMID: 24439385

Hangauer et al (2017) Drug-tolerant persister cancer cells are vulnerable to GPX4 inhibition. Nature 551 247 PMID: 29088702

If you know of a relevant reference for ML 210, please let us know.

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Keywords: ML 210, ML 210 supplier, ML210, Glutathione, peroxidases, GPX4, inhibitors, inhibits, synthetically, lethal, compounds, mutant, RAS, HRAS, ferroptosis, tumor, initiation, cells, persister, drug, resistant, TICs, Ras, GTPases, Ferroptosis, Cancer, Stem, Cells, 6429, Tocris Bioscience

1 Citation for ML 210

Citations are publications that use Tocris products. Selected citations for ML 210 include:

Jeff et al (2020) Human DECR1 is an androgen-repressed survival factor that regulates PUFA oxidation to protect prostate tumor cells from ferroptosis. Elife 9 PMID: 32686647

Do you know of a great paper that uses ML 210 from Tocris? Please let us know.

Reviews for ML 210

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Cancer Metabolism Research Product Guide

Cancer Metabolism Research Product Guide

This product guide reviews some of the main areas in cancer metabolism research and lists around 150 products that can be used to investigate metabolic pathways in cancer including:

RAS Oncoproteins Scientific Review

RAS Oncoproteins Scientific Review

Written by Kirsten L. Bryant, Adrienne D. Cox and Channing J. Der, this review provides a comprehensive overview of RAS protein function and RAS mutations in cancer. Key signaling pathways are highlighted and therapeutic vulnerabilities are explored. This review also includes a detailed section on RAS drug discovery and targeting synthetic lethal interactors of mutant RAS. Compounds available from Tocris are listed.

Cell Cycle & DNA Damage Repair Poster

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. This poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.

Epigenetics in Cancer Poster

Epigenetics in Cancer Poster

This poster summarizes the main epigenetic targets in cancer. The dysregulation of epigenetic modifications has been shown to result in oncogenesis and cancer progression. Unlike genetic mutations, epigenetic alterations are considered to be reversible and thus make promising therapeutic targets.

Pathways for ML 210

Akt Signaling Pathway

Akt Signaling Pathway

The Akt signaling pathway plays a key role in the mediation of protein synthesis, metabolism, proliferation and cell cycle progression. It may be referred to as a 'prosurvival' pathway.
MAPK Signaling Pathway

MAPK Signaling Pathway

The mitogen-activated protein kinase pathway evokes an intracellular signaling cascade in response to extracellular stimuli such as heat and stress. It can influence cell division, metabolism and survival.