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Mpro Tracer

Cat. No. 8126 Submit a Review Datasheet / COA / SDS
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Description: SARS-CoV-2 main protease fluorescent ligand; TR-FRET acceptor
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature (1)
Biological Activity Scientific Data Technical Data Solubility Calculators Datasheets References

Biological Activity for Mpro Tracer

Mpro Tracer is a high affinity (Kd = 51 nM) non-covalent SARS-CoV-2 main protease fluorescent ligand. Mpro Tracer can be used in fluorescent polarisation (FP) assay for inhibitor screening/characterisation. It can be used as a FRET acceptor in time-resolved fluorescence resonance energy-transfer (TR-FRET) experiments. Excitation and emission maxima (λ) are 492 nm and 518 nm.

Scientific Data

Inhibition Activity Fluorescence polarisation assay to determine the inhibitory activity of nirmatrelvir on purified SARS-CoV-2 Main protease protein View Larger

Fluorescence polarisation assay to determine the inhibitory activity of nirmatrelvir on purified SARS-CoV-2 Main protease protein (Mpro) Mpro Tracer and purified Mpro were used at a concentration of 1 μM. A dose response over a range of inhibitor concentrations were measured. Assays were performed in 100 μL wells and left for 1 hour at room temperature before FP measurements were taken. Curve was plotted and fit using graphpad to obtain an IC50 value of 0.74 μM under the assay conditions. Data provided by the Hilgenfeld lab, Lubeck University.

Binding affinity TR-FRET to determine binding affinity of Mpro Tracer View Larger

3CLpro-C10778AA-FITC tracer HTRF assay The Kd of Mpro tracer was measured by varying the tracer concentration against a range of recombinant His6-tagged Mpro concentrations using an HTRF (TR-FRET) assay. MAb Anti-6HIS-Tb GOLD was used as the FRET donor and Mpro Tracer as the FRET acceptor (abs/em = 492nm/518nm). HTRF ratio (520/495) was determined using as Envision plate reader, measurement were recorded after 90 minutes. The Kd of Mpro Tracer was determined to be 51 nM.

Optical Data for Mpro Tracer

Mpro Tracer Dye Spectra
Emission Color Green
λabs 492 nm
λem 518 nm

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Technical Data for Mpro Tracer

M. Wt 909.41
Storage Store at -20°C
Purity ≥95% (HPLC)

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Mpro Tracer

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 9.09 10

Preparing Stock Solutions for Mpro Tracer

The following data is based on the product molecular weight 909.41. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 11 mL 54.98 mL 109.96 mL
0.5 mM 2.2 mL 11 mL 21.99 mL
1 mM 1.1 mL 5.5 mL 11 mL
5 mM 0.22 mL 1.1 mL 2.2 mL

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References for Mpro Tracer

References are publications that support the biological activity of the product.

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Keywords: Mpro Tracer, Mpro Tracer supplier, SARS-Cov-2, cov, sars, Fluorescent, Fluorescence, imaging, polarization, FP, assays, TR-FRET, assay, ligand, protease, acceptor, FRET, coronavirus, antiviral, Other, Probes, Coronavirus, and, Assay, Reagents, 8126, Tocris Bioscience

Citations for Mpro Tracer

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Literature in this Area

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