Home / Product Type / Fluorescence Imaging / Fluorescent Probes and Stains / Other Fluorescent Probes / Mpro Tracer

Mpro Tracer

Cat. No. 8126 Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: SARS-CoV-2 main protease fluorescent ligand; TR-FRET acceptor
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature (1)
Biological Activity Scientific Data Technical Data Solubility Calculators Datasheets References

Biological Activity for Mpro Tracer

Mpro Tracer is a high affinity (Kd = 40 nM) non-covalent SARS-CoV-2 main protease fluorescent ligand. Mpro Tracer can be used in fluorescent polarisation (FP) assay for inhibitor screening/characterisation. It can be used as a FRET acceptor in time-resolved fluorescence resonance energy-transfer (TR-FRET) experiments. Excitation and emission maxima (λ) are 492 nm and 518 nm.

Scientific Data

Inhibition Activity Fluorescence polarisation assay to determine the inhibitory activity of nirmatrelvir on purified SARS-CoV-2 Main protease protein View Larger

Fluorescence polarisation assay to determine the inhibitory activity of nirmatrelvir on purified SARS-CoV-2 Main protease protein (Mpro) Mpro Tracer and purified Mpro were used at a concentration of 1 μM. A dose response over a range of inhibitor concentrations were measured. Assays were performed in 100 μL wells and left for 1 hour at room temperature before FP measurements were taken. Curve was plotted and fit using graphpad to obtain an IC50 value of 0.74 μM under the assay conditions. Data provided by the Hilgenfeld lab, Lubeck University.

Binding affinity TR-FRET to determine binding affinity of Mpro Tracer View Larger

3CLpro-C10778AA-FITC tracer HTRF assay The Kd of Mpro tracer was measured by varying the tracer concentration against a range of recombinant His6-tagged Mpro concentrations using an HTRF (TR-FRET) assay. MAb Anti-6HIS-Tb GOLD was used as the FRET donor and Mpro Tracer as the FRET acceptor (abs/em = 492nm/518nm). HTRF ratio (520/495) was determined using as Envision plate reader, measurement were recorded after 90 minutes. The Kd of Mpro Tracer was determined to be 40 nM.

Optical Data for Mpro Tracer

Mpro Tracer Dye Spectra
Emission Color Green
λabs 492 nm
λem 518 nm

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data for Mpro Tracer

M. Wt 909.41
Storage Store at -20°C
Purity ≥95% (HPLC)

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Mpro Tracer

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 9.09 10

Preparing Stock Solutions for Mpro Tracer

The following data is based on the product molecular weight 909.41. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 11 mL 54.98 mL 109.96 mL
0.5 mM 2.2 mL 11 mL 21.99 mL
1 mM 1.1 mL 5.5 mL 11 mL
5 mM 0.22 mL 1.1 mL 2.2 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

References for Mpro Tracer

References are publications that support the biological activity of the product.

If you know of a relevant reference for Mpro Tracer, please let us know.

View Related Products by Target

Keywords: Mpro Tracer, Mpro Tracer supplier, SARS-Cov-2, cov, sars, Fluorescent, Fluorescence, imaging, polarization, FP, assays, TR-FRET, assay, ligand, protease, acceptor, FRET, coronavirus, antiviral, Other, Probes, Coronavirus, and, Assay, Reagents, 8126, Tocris Bioscience

Citations for Mpro Tracer

Citations are publications that use Tocris products.

Currently there are no citations for Mpro Tracer. Do you know of a great paper that uses Mpro Tracer from Tocris? Please let us know.

Reviews for Mpro Tracer

There are currently no reviews for this product. Be the first to review Mpro Tracer and earn rewards!

Have you used Mpro Tracer?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals