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Description: Fluorescent Cl- indicator; membrane permeable
Chemical Name: 1-(2-Ethoxy-2-oxoethyl)-6-methoxyquinolin-1-ium bromide
Purity: ≥95% (HPLC)
Biological Activity for MQAE
MQAE is a membrane-permeable intracellular chloride ion (Cl-) indicator. The fluorescence intensity of MQAE decreases proportionally as Cl- ions increase. Excitation/emission maxima (λ) are 355 nm and 460 nm respectively. Can be used in fluorescence microscopy and flow cytometry, suitable for use in vivo and in vitro.
Optical Data for MQAE
| λabs | 355 nm |
|---|---|
| λem | 460 nm |
| Extinction Coefficient (ε) | 4850 M-1cm-1 |
| Cell Permeable | Yes |
| pH Sensitivity | Insensitive |
| Application | Confocal imaging, Flow cytometry |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for MQAE
| M. Wt | 326.19 |
| Formula | C14H16BrNO3 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 162558-52-3 |
| PubChem ID | 2762651 |
| InChI Key | DSLLHVISNOIYHR-UHFFFAOYSA-M |
| Smiles | O=C(C[N+]1=CC=CC2=CC(OC)=CC=C21)OCC.[Br-] |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for MQAE
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 3.26 | 10 | |
| water | 3.26 | 10 |
Preparing Stock Solutions for MQAE
The following data is based on the product molecular weight 326.19. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 30.66 mL | 153.28 mL | 306.57 mL |
| 0.5 mM | 6.13 mL | 30.66 mL | 61.31 mL |
| 1 mM | 3.07 mL | 15.33 mL | 30.66 mL |
| 5 mM | 0.61 mL | 3.07 mL | 6.13 mL |
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Product Datasheets for MQAE
References for MQAE
References are publications that support the biological activity of the product.
Verkman et al (1989) Synthesis and characterization of improved chloride-sensitive fluorescent indicators for biological applications. Anal.Biochem. 178 355 PMID: 2751097
Wan et al (2019) An ATP-regulated ion transport nanosystem for homeostatic perturbation therapy and sensitizing photodynamic therapy by autophagy inhibition of tumors. ACS Cent.Sci. 5 327 PMID: 30834321
If you know of a relevant reference for MQAE, please let us know.
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
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