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NBD-PE

Cat. No. 7538 Submit a Review Datasheet / COA / SDS
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Description: Green-fluorescent label for lysosomal lipid bodies. Used for: Application: confocal microscopy
Chemical Name: Triethylammonium (R)-2,3-bis(palmitoyloxy)propyl(2-((7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)amino)ethyl)phosphate
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for NBD-PE

Key information: NBD-PE is a fluorescent label for lysosomal lipid bodies.

Used for: quantitatively measure phospholipidosis, which is useful to screen drugs for SARS-CoV-2 repurposing. NBD-PE can also be used to label giant unilamellar vesicles (GUV).

Application: confocal microscopy, fluorescence recovery after photobleaching (FRAP).

Properties and Photophysical Data: It consists of a single tail (16:0) phospholipid derivative with an NBD fluorophore conjugated to the head group. Excitation and emission maxima (λ) are 463 nm and 536 nm, respectively, extinction coefficient = 22,000 M-1cm-1.

Optical Data for NBD-PE

Emission Color Green
λabs 463 nm
λem 536 nm
Extinction Coefficient (ε) 22000 M-1cm-1
Application Confocal Microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for NBD-PE

M. Wt 956.26
Formula C43H75N4O11P.C6H15N
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 178119-00-1
InChI Key LEJHDNAGUHKMLR-GKEJWYBXSA-N
Smiles CC[N+](CC)([H])CC.CCCCCCCCCCCCCCCC(OC[C@@H](OC(CCCCCCCCCCCCCCC)=O)COP([O-])(OCCNC1=CC=C(N(=O)=O)C2=NON=C12)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for NBD-PE

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
methanol 0.48 0.5 with sonication

Preparing Stock Solutions for NBD-PE

The following data is based on the product molecular weight 956.26. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.005 mM 209.15 mL 1045.74 mL 2091.48 mL
0.025 mM 41.83 mL 209.15 mL 418.3 mL
0.05 mM 20.91 mL 104.57 mL 209.15 mL
0.25 mM 4.18 mL 20.91 mL 41.83 mL

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References for NBD-PE

References are publications that support the biological activity of the product.

Morelli et al (2006) Validation of an in vitroscreen for phospholipidosis using a high-content biology platform. Cell Biol.Toxicol. 22 15 PMID: 16463016

Gum et al (2001) Analysis of two matrix metalloproteinase inhibitors and their metabolites for induction of phospholipidosis in rat and human hepatocytes. Biochem.Pharmacol. 62 1661 PMID: 11755120

Li et al (2020) Programmed magnetic manipulation of vesicles into spatially coded prototissue architectures arrays. Nat.Commun. 11 232 PMID: 31932592

Tummino et al (2021) Drug-induced phospholipidosis confounds drug repurposing for SARS-CoV-2. Science 373 541 PMID: 34326236

If you know of a relevant reference for NBD-PE, please let us know.

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Citations for NBD-PE

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Literature in this Area

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

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