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Submit ReviewNicotinamide
Description: PARP-1 inhibitor
Chemical Name: Pyridine-3-carboxamide
Purity: ≥99% (HPLC)
Biological Activity for Nicotinamide
Nicotinamide is an anti-inflammatory agent. Inhibitor of poly(ADP-ribose) polymerase (PARP-1) enzymes. NAD+ precursor. Promotes differentiation of mesenchymal stem cells to insulin producing cells when used in combination with growth factors and high glucose concentration. Nicotinamide also acts as a SIRT1 inhibitor and promotes the expansion of hematopoietic progenitor cells
Nicotinamide synthesized to Ancillary Material Grade also available.
For more information about how Nicotinamide may be used, see our protocol:
3D Culture of Lung Alveolar CellsCompound Libraries for Nicotinamide
Nicotinamide is also offered as part of the Tocriscreen Epigenetics Library and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.
Technical Data for Nicotinamide
| M. Wt | 122.12 |
| Formula | C6H6N2O |
| Storage | Store at RT |
| Purity | ≥99% (HPLC) |
| CAS Number | 98-92-0 |
| PubChem ID | 936 |
| InChI Key | DFPAKSUCGFBDDF-UHFFFAOYSA-N |
| Smiles | NC(C1=CC=CN=C1)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Nicotinamide
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 12.21 | 100 |
Preparing Stock Solutions for Nicotinamide
The following data is based on the product molecular weight 122.12. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 8.19 mL | 40.94 mL | 81.89 mL |
| 5 mM | 1.64 mL | 8.19 mL | 16.38 mL |
| 10 mM | 0.82 mL | 4.09 mL | 8.19 mL |
| 50 mM | 0.16 mL | 0.82 mL | 1.64 mL |
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Product Datasheets for Nicotinamide
References for Nicotinamide
References are publications that support the biological activity of the product.
Suzuki et al (2010) Protective effect of nicotinamide against poly(ADP-ribose) polymerase-1-mediated astrocyte death depends on its transporter-mediated uptake. Life.Sci. 86 676 PMID: 20188745
Cimadamore et al (2009) Nicotinamide rescues human embryonic stem cell-derived neuroectoderm from parthanatic cell death. Stem Cells. 27 1772 PMID: 19544437
Czubak et al (2014) A modified method of Ins producing cells' generation from bone marrow-derived mesenchymal stem cells. J.Diabetes Res. 2014 628591 PMID: 25405207
Sato et al (2015) SnapShot: Growing organoids from stem cells. Cell 161 1700 PMID: 26091044
Bartfeld et al (2015) In vitro expansion of human gastric epithelial stem cells and their responses to bacterial infection. Gastroenterology 148 126 PMID: 25307862
Jung et al (2011) Isolation and in vitro expansion of human colonic stem cells. Nat.Med. 17 1227 PMID: 21892181
Boj et al (2015) Organoid models of human and mouse ductal pancreatic cancer. Cell 160 324 PMID: 25557080
Peled et al (2012) Nicotinamide, a SIRT1 inhibitor, inhibits differentiation and facilitates expansion of hematopoietic progenitor cells with enhanced bone marrow homing and engraftment. Exp.Hematol. 40 342 PMID: 22198152
If you know of a relevant reference for Nicotinamide, please let us know.
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Keywords: Nicotinamide, Nicotinamide supplier, Nicotinamide, inhibits, poly(ADP-ribose), polymerase-1, PARP-1, inhibitors, anti-inflammatory, organoids, hematopoietic, haematopoietic, stem, cells, HSC, progenitors, SIRT1, Poly(ADP-ribose), Polymerase, Mesenchymal, Stem, Cells, Organoids, Hematopoietic, Class, III, HDACs, (Sirtuins), 4106, Tocris Bioscience
6 Citations for Nicotinamide
Citations are publications that use Tocris products. Selected citations for Nicotinamide include:
Emanuela et al (2020) Cell-type-specific regulation of neuronal intrinsic excitability by macroautophagy. Elife 9 PMID: 31913125
Jun et al (2017) Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency. Cell 169 243-257.e25 PMID: 28388409
Thomas C et al (2019) Direct Reprogramming of Human Neurons Identifies MARCKSL1 as a Pathogenic Mediator of Valproic Acid-Induced Teratogenicity. Cell Stem Cell 25 103-119.e6 PMID: 31155484
Mark R et al (2019) Inhibition of APE1-endonuclease activity affects cell metabolism in colon cancer cells via a p53-dependent pathway. DNA Repair (Amst) 82 102675 PMID: 31450087
Mihee M et al (2019) Evaluation of Compound Activity in Primary Human Intestinal Organoids Using Gene Expression and Histology. Curr Protoc Pharmacol 85 e54 PMID: 30920154
Steven L et al (2022) INF2-mediated actin filament reorganization confers intrinsic resilience to neuronal ischemic injury. Nat Commun 13 6037 PMID: 36229429
Do you know of a great paper that uses Nicotinamide from Tocris? Please let us know.
Reviews for Nicotinamide
Average Rating: 5 (Based on 1 Review.)
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Nicotinamide test.
By
Anonymous on 08/21/2024
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: pancreatic tumor
We used this reagent for human pancreatic tumor organoid culture by using 10 mM treatment.
Protocols for Nicotinamide
The following protocol features additional information for the use of Nicotinamide (Cat. No. 4106).
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
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Cell Cycle and DNA Damage Research Product Guide
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Cell Cycle & DNA Damage Repair Poster
In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. This poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.