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Submit ReviewNile Red
Description: Fluorogenic lipid membrane dye. Used for: intracellular lipid droplets and neutral lipids staining, suitable for live cell imaging. Application: fluorescence microscopy
Alternative Names: Nile blue oxazone
Chemical Name: 9-(Diethylamino)-5H-benzo[a]phenoxazin-5-one
Purity: ≥98% (HPLC)
Biological Activity for Nile Red
Key information: Nile red is a fluorogenic lipid membrane dye.
Used for: staining of intracellular lipid droplets and neutral lipids. It is suitable for long-term monitoring of lipid dynamics in living cells.
Application: fluorescence microscopy.
Properties and Photophysical Data: Nile red fluoresces in the hydrophobic environment of lipid membranes. Non-fluorescent in polar environments (e.g. water). Excitation and emission maxima (λ) are 552 nm and 636 nm in methanol, respectively.
Optical Data for Nile Red
| Emission Color | Red |
|---|---|
| λabs | 552 nm |
| λem | 636 nm |
| Application | Fluorescence Microscopy, Monitoring of Lipid Dynamics in Living Cells |
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Technical Data for Nile Red
| M. Wt | 318.38 |
| Formula | C20H18N2O2 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 7385-67-3 |
| PubChem ID | 65182 |
| InChI Key | VOFUROIFQGPCGE-UHFFFAOYSA-N |
| Smiles | CCN(C1=CC2=C(N=C3C(O2)=CC(C4=CC=CC=C34)=O)C=C1)CC |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Nile Red
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 3.18 | 10 |
Preparing Stock Solutions for Nile Red
The following data is based on the product molecular weight 318.38. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 31.41 mL | 157.05 mL | 314.09 mL |
| 0.5 mM | 6.28 mL | 31.41 mL | 62.82 mL |
| 1 mM | 3.14 mL | 15.7 mL | 31.41 mL |
| 5 mM | 0.63 mL | 3.14 mL | 6.28 mL |
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Product Datasheets for Nile Red
References for Nile Red
References are publications that support the biological activity of the product.
Maes et al (2017) A rapid-screening approach to detect and quantify microplastics based on fluorescent tagging with Nile red. Sci.Rep 16 44501 PMID: 28300146
Zhanghao et al (2020) High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes. Nat.Commun. 11 5890 PMID: 33208737
Uribe-Etxebarria et al (2019) Wnt signaling reprograms metabolism in dental pulp stem cells. J.Cell Physiol. 234 13068 PMID: 30549037
If you know of a relevant reference for Nile Red, please let us know.
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Keywords: Nile Red, Nile Red supplier, fluorogenic, lipids, membranes, red, dye, Nile, blue, oxazone, Fluorescent, Lipid, Probes, and, Cell, Membrane, Stains, 7387, Tocris Bioscience
Citations for Nile Red
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
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- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
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- TSA Reagents for Enhancing IHC, ICC & FISH Signals