Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewNocodazole
Description: Microtubule inhibitor
Chemical Name: [5-(2-Thienylcarbonyl)-1H-benzimidazol-2-yl]carbonic acid, methyl ester
Purity: ≥95% (HPLC)
Biological Activity for Nocodazole
Nocodazole is a microtubule inhibitor; inhibits mitosis. Also inhibits autophagosome-lysosome fusion. Enhances homology-directed repair (HDR) efficiency 9 to 31% (depending on cell cycle phase) and increases Cas9-mediated gene editing frequencies.
Technical Data for Nocodazole
| M. Wt | 301.32 |
| Formula | C14H11N3O3S |
| Storage | Store at RT |
| Purity | ≥95% (HPLC) |
| CAS Number | 31430-18-9 |
| PubChem ID | 4122 |
| InChI Key | KYRVNWMVYQXFEU-UHFFFAOYSA-N |
| Smiles | COC(=O)NC1=NC2=CC(=CC=C2N1)C(=O)C1=CC=CS1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Nocodazole
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 15.07 | 50 |
Preparing Stock Solutions for Nocodazole
The following data is based on the product molecular weight 301.32. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 6.64 mL | 33.19 mL | 66.37 mL |
| 2.5 mM | 1.33 mL | 6.64 mL | 13.27 mL |
| 5 mM | 0.66 mL | 3.32 mL | 6.64 mL |
| 25 mM | 0.13 mL | 0.66 mL | 1.33 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
Product Datasheets for Nocodazole
References for Nocodazole
References are publications that support the biological activity of the product.
Vasquez et al (1997) Nanomolar concentrations of nocodazole alter microtubule dynamic instability in vivo and in vitro. Mol.Biol.Cell 8 973 PMID: 9201709
Webb et al (2004) Microtubule disruption inhibits autophagosome-lysosome fusion: implications for studying the roles of aggresomes in polyglutamine diseases. Int.J.Biochem.Cell Biol. 36 2541 PMID: 15325591
Mizushima et al (2010) Methods in mammalian autophagy research. Cell 140 313 PMID: 20144757
Lin et al (2014) Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery. Elife 3 e04766 PMID: 25497837
If you know of a relevant reference for Nocodazole, please let us know.
View Related Products by Target
Keywords: Nocodazole, Nocodazole supplier, Microtubule, inhibitors, inhibits, Tau, Tubulin, Microtubules, Mitosis, crispr, cas9, homology-directed, repair, HDR, Autophagy, CRISPR, Reagents, 1228, Tocris Bioscience
12 Citations for Nocodazole
Citations are publications that use Tocris products. Selected citations for Nocodazole include:
Bernier et al (2019) Nanoscale Surveillance of the Brain by Microglia via cAMP-Regulated Filopodia. Cell Rep 27 2895 PMID: 31167136
Melemedjian et al (2014) Local translation and retrograde axonal transport of CREB regulates IL-6-induced nociceptive plasticity. Mol Pain 10 45 PMID: 24993495
Seese et al (2012) LTP induction translocates cortactin at distant synapses in wild-type but not Fmr1 knock-out mice. J Neurosci 32 7403 PMID: 22623686
Lin et al (2015) Drosophila homologue of Diaphanous 1 (DIAPH1) controls the metastatic potential of colon cancer cells by regulating microtubule-dependent adhesion. Nat Commun 6 18577 PMID: 26124177
Tauchman et al (2015) Stable kinetochore-microtubule attachment is sufficient to silence the spindle assembly checkpoint in human cells. Nat Cell Biol 6 10036 PMID: 26620470
Hutchins and Wray (2014) Capture of microtubule plus-ends at the actin cortex promotes axophilic neuronal migration by enhancing microtubule tension in the leading process. Methods Mol Biol 8 400 PMID: 25505874
Schapitz et al (2010) Neuroligin 1 is dynamically exchanged at postsynaptic sites. Oncotarget 30 12733 PMID: 20861378
Macheret and Halazonetis (2018) Intragenic origins due to short G1 phases underlie oncogene-induced DNA replication stress. Nature 555 112 PMID: 29466339
Romero et al (2018) Damage-induced reactive oxygen species enable zebrafish tail regeneration by repositioning of Hedgehog expressing cells. Nat Commun 9 4010 PMID: 30275454
Guo et al (2016) Site-specific proteasome phosphorylation controls cell proliferation and tumorigenesis. Front Cell Neurosci 18 202 PMID: 26655835
Rood et al (2011) Methods for purification of proteins associated with cellular poly(ADP-ribose) and PARP-specific poly(ADP-ribose). Anal Biochem 780 153 PMID: 21870260
Paonessa et al (2019) Microtubules Deform the Nuclear Membrane and Disrupt Nucleocytoplasmic Transport in Tau-Mediated Frontotemporal Dementia. Cell Rep 26 582 PMID: 30650353
Do you know of a great paper that uses Nocodazole from Tocris? Please let us know.
Reviews for Nocodazole
Average Rating: 5 (Based on 1 Review.)
Have you used Nocodazole?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
Microtubule disruption.
By
Anonymous on 01/30/2018
Assay Type: Ex Vivo
Species: Mouse
Cell Line/Tissue: Brain slice
Used in brain slcies to disturb microtubule dynamics. Effect on cell shape was observed within minutes
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Cell Cycle and DNA Damage Research Product Guide
This product guide provides a review of the cell cycle and DNA damage research area and lists over 150 products, including research tools for:
- Cell Cycle and Mitosis
- DNA Damage Repair
- Targeted Protein Degradation
- Ubiquitin Proteasome Pathway
- Chemotherapy Targets