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NR12S

Cat. No. 7509 Submit a Review Datasheet / COA / SDS
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Description: Fluorogenic lipid membrane dye. Used for: staining lipid at the outer leaflet of vesicles and cell membranes, suitable for live cell imaging. Application: fluorescence microscopy
Chemical Name: 3-((3-((9-(Diethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl)oxy)propyl)(dodecyl)(methyl)ammonio)propane-1-sulfonate
Purity: ≥95% (HPLC)
Datasheet
Citations
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Literature (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for NR12S

Key information: NR12S is a fluorogenic lipid membrane dye that stains the outer leaflet of lipid vesicles and cell membranes. Suitable for live cell imaging.

Used for: monitoring cholesterol and lipid order selectively at the outer leaflet of cell membranes.

Application: fluorescence microscopy.

Properties and Photophysical Data: NR12S is a conjugation of the Nile Red fluorophore with a long alkyl chain and a zwitterionic group, allowing strong interactions with the lipid membranes. Excitation and emission maxima (λ) are 554 nm and 627 nm in DMSO, respectively; quantum yield = 0.55.

Optical Data for NR12S

NR12S Dye Spectra
Emission Color Orange
λabs 554 nm
λem 627 nm
Quantum Yield (φ) 0.55
Closest Laser line 532 nm
Application Monitoring of Lipid Dynamics in Living Cells, Fluorescence Microscopy
Alternative For Nile Red

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Technical Data for NR12S

M. Wt 695.96
Formula C39H57N3O6S
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 1221739-85-0
PubChem ID 146165932
InChI Key MJGSHKTVWQIWAA-UHFFFAOYSA-N
Smiles CCCCCCCCCCCC[N+](CCCOC1=CC=C2C(C=C3OC4=CC(N(CC)CC)=CC=C4N=C3C2=C1)=O)(CCCS([O-])(=O)=O)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for NR12S

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 6.96 10

Preparing Stock Solutions for NR12S

The following data is based on the product molecular weight 695.96. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 14.37 mL 71.84 mL 143.69 mL
0.5 mM 2.87 mL 14.37 mL 28.74 mL
1 mM 1.44 mL 7.18 mL 14.37 mL
5 mM 0.29 mL 1.44 mL 2.87 mL

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References for NR12S

References are publications that support the biological activity of the product.

Kucherak et al (2010) Switchable nile red-based probe for cholesterol and lipid order at the outer leaflet of biomembranes. J.Am.Chem.Soc. 132 4907 PMID: 20225874

If you know of a relevant reference for NR12S, please let us know.

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Keywords: NR12S, NR12S supplier, fluorogenic, probe, lipid, cholesterol, membranes, orange, dye, Nile, fluorescence, Fluorescent, Lipid, Probes, and, Cell, Membrane, Stains, 7509, Tocris Bioscience

Citations for NR12S

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Literature in this Area

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

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