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Submit ReviewPARPi-FL
Description: Potent fluorescent PARP inhibitor; cell permeable
Alternative Names: AZD2281-BDY FL,Olaparib-BDY FL
Chemical Name: (T-4)-[4-[[3-[[4-[3-[5-[(3,5-Dimethyl-2H-pyrrol-2-ylidene-κN)methyl]-1H-pyrrol-2-yl-κN-1-oxopropyl]-1-piperazinyl]carbonyl]-4-fluorophenyl]methyl]-1(2H)-phthalazinonato]difluoroboron
Purity: ≥90% (HPLC)
Biological Activity for PARPi-FL
PARPi-FL is a fluorescent potent PARP inhibitor (IC50 = 12 nM). Composed of a PARP inhibitor conjugated to BDY FL, SE (Cat. No. 5465). Colocalizes with PARP immunostaining in multiple cancer cell lines in vivo and accumulates in tumor cells and tumor associated macrophages. Enables high temporal and subcellular spatial resolution of drug distribution. Emission max = 525 nm. Cell permeable.
Optical Data for PARPi-FL
| Emission Color | Green |
|---|---|
| λabs | 502 nm |
| λem | 510 nm |
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Technical Data for PARPi-FL
| M. Wt | 640.46 |
| Formula | C34H32BF3N6O3 |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 1380359-84-1 |
| PubChem ID | 70697686 |
| InChI Key | IGUTVNUEFKPBGK-UHFFFAOYSA-N |
| Smiles | CC1=CC(C)=[N+]2C1=CC3=CC=C(N3[B-]2(F)F)CCC(N4CCN(C(C5=CC(CC6=NNC(C7=CC=CC=C67)=O)=CC=C5F)=O)CC4)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for PARPi-FL
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 6.4 | 10 |
Preparing Stock Solutions for PARPi-FL
The following data is based on the product molecular weight 640.46. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 15.61 mL | 78.07 mL | 156.14 mL |
| 0.5 mM | 3.12 mL | 15.61 mL | 31.23 mL |
| 1 mM | 1.56 mL | 7.81 mL | 15.61 mL |
| 5 mM | 0.31 mL | 1.56 mL | 3.12 mL |
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Product Datasheets for PARPi-FL
References for PARPi-FL
References are publications that support the biological activity of the product.
Thurber et al (2013) Single-cell and subcellular pharmacokinetic imaging allows insight into drug action in vivo. Nat.Commun. 4 1504 PMID: 23422672
Thurber et al (2014) Effect of small-molecule modification on single-cell pharmacokinetics of PARP inhibitors. Mol.Cancer Ther. 13 986 PMID: 24552776
Dubach et al (2017) Quantitating drug-target engagement in single cells in vitro and in vivo. Nat.Chem.Biol. 13 168 PMID: 27918558
If you know of a relevant reference for PARPi-FL, please let us know.
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
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- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals