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Submit ReviewPepstatin A Janelia Fluor® 526
Description: Fluorogenic green-emitting lysosome tracker and stain
Alternative Names: Pepstatin A JF526
Chemical Name: 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-(((6S,9S,12S,13S,17S,20S,21S)-13,21-dihydroxy-12,20-diisobutyl-6,9-diisopropyl-2,17-dimethyl-4,7,10,15,18,23-hexaoxo-5,8,11,16,19,24-hexaazatriacontan-30-yl)carbamoyl)benzoate trifluoroacetate
Purity: ≥90% (HPLC)
Biological Activity for Pepstatin A Janelia Fluor® 526
Pepstatin A Janelia Fluor® 526 is a fluorogenic green-emitting lysosome tracker and stain. No wash step is required when used for multicolor microscopy experiments. Pepstatin A Janelia Fluor® 526 can be used for live cell imaging, in confocal microscopy and super-resolution microscopy techniques including 3D-SIM (structured illumination microscopy) and STED, as well as two-color lattice light-sheet microscopy (lls). Excitation maximum = 530 nm, emission maximum = 549 nm.
To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.
Scientific Data
Confocal Microscopy
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Application of Pepstatin A Janelia Fluor® 526 in live U2OS cells. Live U2OS cells were stained with Pepstatin A Janelia Fluor® 526 (2 µM) for 1 hour with Verapamil (10 µM) and imaged without an intermediate washing step on a LSM980 confocal microscope (Zeiss) using the following configuration: excitation: 514 nm / emission: 526–615 nm. Image kindly provided by Prof. Luke Lavis, Howard Hughes Medical Institute, Janelia Research Campus.
Confocal Microscopy
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Application of Pepstatin A Janelia Fluor® 526 in fixed U2OS cells. Fixed U2OS cells were stained with Pepstatin A Janelia Fluor® 526 (2 µM) for 1 hour in 0.1 M sodium acetate buffer pH 4.5. Cells were imaged on a LSM980 confocal microscope (Zeiss) using the following configuration: excitation: 514 nm / emission: 526–615 nm. Image kindly provided by Prof. Luke Lavis, Howard Hughes Medical Institute, Janelia Research Campus
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Pepstatin A Janelia Fluor® 526
| Emission Color | Yellow |
|---|---|
| λabs | 530 nm |
| λem | 549 nm |
| Extinction Coefficient (ε) | 118,000 M-1cm-1 |
| Quantum Yield (φ) | 0.87 |
| Application | Confocal Microscopy, STED, 3D-SIM, Lattice Light-sheet Microscopy |
| Alternative For | Lysotracker™- Lysotracker is a registered trademark of Thermo Fisher |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for Pepstatin A Janelia Fluor® 526
| M. Wt | 1442.53 |
| Formula | C67H91F6N9O12.CF3CO2H |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 2410614-07-0 |
| InChI Key | JONSFJPZCPEZFP-WKVYEHBGSA-N |
| Smiles | OC(C(F)(F)F)=O.CC(C[C@@H]([C@H](CC(NCCCCCCNC(C1=CC(C2=C3C=C(/C(C=C3OC4=C2C=C(C(N5CC(F)(C5)F)=C4)F)=[N+]6CC(F)(C\6)F)F)=C(C([O-])=O)C=C1)=O)=O)O)NC([C@@H](NC(C[C@@H]([C@@H](NC([C@H](C(C)C)NC([C@H](C(C)C)NC(CC(C)C)=O)=O)=O)CC(C)C)O)=O)C)=O)C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Pepstatin A Janelia Fluor® 526
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 14.43 | 10 |
Preparing Stock Solutions for Pepstatin A Janelia Fluor® 526
The following data is based on the product molecular weight 1442.53. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 6.93 mL | 34.66 mL | 69.32 mL |
| 0.5 mM | 1.39 mL | 6.93 mL | 13.86 mL |
| 1 mM | 0.69 mL | 3.47 mL | 6.93 mL |
| 5 mM | 0.14 mL | 0.69 mL | 1.39 mL |
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Product Datasheets for Pepstatin A Janelia Fluor® 526
References for Pepstatin A Janelia Fluor® 526
References are publications that support the biological activity of the product.
Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787
Chen et al (2000) Probing the cathepsin D using a BODIPY FL-pepstatin A: applications in fluorescence polarization and microscopy. J.Biochem.Biophys.Methods 42 137 PMID: 10737220
If you know of a relevant reference for Pepstatin A Janelia Fluor® 526, please let us know.
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Citations for Pepstatin A Janelia Fluor® 526
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Reviews for Pepstatin A Janelia Fluor® 526
Average Rating: 4 (Based on 1 Review.)
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Specific dye.
By
Karthik Krishnamurthy on 08/03/2021
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: astrocytes
labeling of lysosomes in astrocytes under basal conditions
