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Submit ReviewPhalloidin-Janelia Fluor® 646
Description: Red-fluorescent F-actin probe. Binds and labels F-actin. Used for: F-actin labelling. Application: confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED, flow cytometry
Alternative Names: Phalloidin-JF646
Purity: ≥90% (HPLC)
Biological Activity for Phalloidin-Janelia Fluor® 646
Key information: Phalloidin-Janelia Fluor® 646 is a red fluorescent F-actin probe. It binds and labels specifically F-actin.
Used for: Phalloidin-Janelia Fluor® 646 can be used to visualize and quantify F-actin in tissue sections, cell cultures or cell-free experiments with optimal results in formaldehyde-fixed and permeabilized cells.
Application: confocal microscopy, super-resolution microscopy (SRM) including dSTORM and STED and flow cytometry.
Properties and Photophysical Data: Phalloidin-Janelia Fluor® 646 is composed of the F-actin probe, Phalloidin (Cat. No. 4535), conjugated to Janelia Fluor® 646 (Cat. No. 6148). Excitation and emission maxima (λ) are 646 nm and 664 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1.
Scientific Data
Confocal Microscopy
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Application of Phalloidin-Janelia Fluor® 646. HeLa cells were fixed for 10 minutes with 4% formaldehyde in PBS and permeabilized with PBS/0.25% Triton X-100 for 6 minutes. An additional post-fixation step was performed twice with 1% formaldehyde in PBS followed by 3 washes with PBS. Phalloidin Janelia Fluor® 646 was prepared as a 10 μM stock solution diluted in methanol and diluted to a working concentration of 20 nM in PBS. Stained cells were imaged with a Leica SP8X WLL upright confocal microscope. Image kindly provided by Brigitte Bergner, Steffen Dietzel, Core Facility Bioimaging at the Biomedical Center of the Ludwig-Maximilians-Universität München.
Confocal Microscopy
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Application of Phalloidin-Janelia Fluor® 646. HeLa cells were fixed for 10 minutes with 4% formaldehyde in PBS and permeabilized with PBS/0.25% Triton X-100 for 6 minutes. An additional post-fixation step was performed twice with 1% formaldehyde in PBS followed by 3 washes with PBS. Phalloidin Janelia Fluor® 646 was prepared as a 10 μM stock solution diluted in methanol and diluted to a working concentration of 20 nM in PBS. Stained cells were imaged with a Leica SP8X WLL upright confocal microscope. Image kindly provided by Brigitte Bergner, Steffen Dietzel, Core Facility Bioimaging at the Biomedical Center of the Ludwig-Maximilians-Universität München.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Phalloidin-Janelia Fluor® 646
| Emission Color | Red |
|---|---|
| Brightness | |
| λabs | 646 nm |
| λem | 664 nm |
| Extinction Coefficient (ε) | 152,000 M-1cm-1 |
| Quantum Yield (φ) | 0.54 |
| Closest Laser line | 640 nm |
| Application | Confocal Imaging, dSTORM, STED, Flow Cytometry |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for Phalloidin-Janelia Fluor® 646
| M. Wt | 1266.51 |
| Formula | C64H75N11O13SSi |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| InChI Key | AOXMEZGPCIYGOB-BNAQDRCBSA-N |
| Smiles | O=C([C@H](NC([C@H](C)NC([C@H](C[C@](O)(C)CNC(C1=CC=C(C([O-])=O)C(C2=C(C=C/3)C([Si](C)(C)C4=CC(N5CCC5)=CC=C42)=CC3=[N+]6CCC\6)=C1)=O)N7)=O)=O)[C@H](C)O)N[C@H]8CSC(N9)=C(C[C@H](NC([C@H](C)NC([C@@H]%10C[C@@H](O)CN%10C8=O)=O)=O)C7=O)C%11=C9C=CC=C%11 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Phalloidin-Janelia Fluor® 646
References for Phalloidin-Janelia Fluor® 646
References are publications that support the biological activity of the product.
Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods 12 244 PMID: 25599551
Cooper et al (1987) Effects of cytochalasin and phalloidin on actin. J.Cell.Biol. 105 1473 PMID: 3312229
If you know of a relevant reference for Phalloidin-Janelia Fluor® 646, please let us know.
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Citations for Phalloidin-Janelia Fluor® 646
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