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Description: Red-orange fluorescent cytoskeleton stain. Binds and labels F-actin. Used for: F-actin staining. Application: fluorescent microscopy, immunofluorescence (IHC, ICC)
Chemical Name: 7-[(4R)-5-[[[[4-[3,6-Bis(dimethylamino)xanthylium-9-yl]-3-carboxyphenyl]amino]thioxomethyl]amino]-4-hydroxy-L-leucine]phalloidin
Purity: ≥90% (HPLC)
Biological Activity for Phalloidin-TRITC
Key information: Phalloidin-TRITC is a red-orange fluorescent cytoskeleton stain. It binds and labels specifically F-actin.
Used for: Phalloidin-TRITC can be used to visualize and quantify F-actin in tissue sections, cell cultures or cell-free experiments with optimal results in formaldehyde-fixed and permeabilized cells.
Application: fluorescent microscopy, immunofluorescence (IHC, ICC).
Properties and Photophysical Data: Phalloidin-TRITC is composed of the F-actin probe, Phalloidin (Cat. No. 4535), conjugated to Tetramethylrhodamine isothiocyanate (TRITC) dye. Phalloidin binds F-actin with high selectivity allowing Phalloidin-TRITC to stain actin with high-contrast discrimination. Excitation and emission maxima (λ) are 540 nm and 565 nm, respectively.
We suggest making up a stock solution of this product by dissolving the vial contents in 1.5 mL methanol or DMSO. Please refer to the protocol for further information on using this product.
Scientific Data
Fluorescence Imaging
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Applications in HeLa Cells for Phalloidin-TRITC. Application of Phalloidin-TRITC: HeLa cells were labeled with 150 nM Phalloidin-TRITC for 20 minutes. Counterstained with DAPI (Catalog # 5748).
Technical Data for Phalloidin-TRITC
| M. Wt | 1231.4 |
| Formula | C60H70N12O13S2 |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 915013-10-4 |
| Smiles | C[C@H](O)[C@H]1NC(=O)C(C)NC(=O)[C@H](C[C@@](C)(O)CNC(=S)NC2=CC(C([O-])=O)=C(C=C2)C2=C3C=CC(C=C3OC3=C2C=CC(=C3)N(C)C)=[N+](C)C)NC(=O)[C@@H]2CC3=C(NC4=C3C=CC=C4)SC[C@@H](NC1=O)C(=O)N1C[C@H](O)C[C@H]1C(=O)N[C@@H](C)C(=O)N2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Phalloidin-TRITC
References for Phalloidin-TRITC
References are publications that support the biological activity of the product.
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Keywords: Phalloidin-TRITC, Phalloidin-TRITC supplier, red, orange, fluorescent, cytoskeleton, actin, filament, stain, probe, Organelle, and, Cell, Structure, Stains, Fluorescent, Actin, Probes, 5783, Tocris Bioscience
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Protocols for Phalloidin-TRITC
The following protocol features additional information for the use of Phalloidin-TRITC (Cat. No. 5783).
Literature in this Area
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
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