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SAHA-FITC

Cat. No. 7970 Submit a Review Datasheet / COA / SDS
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Description: Fluorescent HDAC1/2 probe; suitable for use in TR-FRET and FP assays
Chemical Name: 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)-5-(3-(4-(8-(hydroxyamino)-8-oxooctanamido)benzyl)thioureido)benzoic acid
Purity: ≥90% (HPLC)
Datasheet
Citations
Reviews
Literature (2)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for SAHA-FITC

SAHA-FITC is a fluorescent HDAC1/2 probe for use as a TR-FRET acceptor for CoraFluor labeling and in fluorescence polarization binding assays. Binds non-selectively to HDAC1 and HDAC2 (Kd values are 56 nM and 132 nM respectively; Ki = 1.5 nM).

Optical Data for SAHA-FITC

SAHA-FITC Dye Spectra
Emission Color Green
λabs 495 nm
λem 525 nm

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for SAHA-FITC

M. Wt 682.75
Formula C36H34N4O8S
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 1160823-10-8
PubChem ID 44123666
InChI Key GQKWRDWQZXNRAB-UHFFFAOYSA-N
Smiles OC(C=C1OC2=C3)=CC=C1C(C4=CC=C(NC(NCC5=CC=C(NC(CCCCCCC(NO)=O)=O)C=C5)=S)C=C4C(O)=O)=C2C=CC3=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for SAHA-FITC

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 6.83 10

Preparing Stock Solutions for SAHA-FITC

The following data is based on the product molecular weight 682.75. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 14.65 mL 73.23 mL 146.47 mL
0.5 mM 2.93 mL 14.65 mL 29.29 mL
1 mM 1.46 mL 7.32 mL 14.65 mL
5 mM 0.29 mL 1.46 mL 2.93 mL

Molarity Calculator

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Reconstitution Calculator

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Dilution Calculator

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References for SAHA-FITC

References are publications that support the biological activity of the product.

Kral et al (2014) Divergent kinetics differentiate the mechanism of action of two HDAC inhibitors. Biochemistry 53 725 PMID: 24450491

Payne and Mazitschek (2022) Resolving the deceptive isoform and complex selectivity of HDAC1/2 inhibitors. Cell Chem.Biol. 29 1140 PMID: 35298895

If you know of a relevant reference for SAHA-FITC, please let us know.

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Keywords: SAHA-FITC, SAHA-FITC supplier, SAHAFITC, SAHA, FITC, TR-FRET, TRFRET, fluorescence, HDAC, histone, deacetylase, fluorescein, isothiocyanate, and, FP, Assay, Reagents, Class, I, HDACs, 7970, Tocris Bioscience

Citations for SAHA-FITC

Citations are publications that use Tocris products.

Currently there are no citations for SAHA-FITC. Do you know of a great paper that uses SAHA-FITC from Tocris? Please let us know.

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Literature in this Area

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