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Submit ReviewSulfidefluor 7 AM
Description: H2S fluorescent probe
Chemical Name: N-[2-[(Acetyloxy)methoxy]-2-oxoethyl]-N-[(3',6'-diazido-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)carbonyl]glycine (acetyloxy)methyl ester
Biological Activity for Sulfidefluor 7 AM
Sulfidefluor 7 AM is a reported to be a hydrogen sulphide (H2S) fluorescent probe. Contains an acetoxymethyl ester, which enhances cell permeability and is hydrolyzed inside the cell to trap the probe. Used to visualize VEGF-induced H2S production in human umbilical vein endothelial cells (HUVECS). Excitation wavelength ~ 495 nm; emission maximum ~ 520 nm.
Licensing Information
Sold under license from the Regents of the University of California
Optical Data for Sulfidefluor 7 AM
| Emission Color | Green |
|---|---|
| λabs | 495 nm |
| λem | 520 nm |
| Cell Permeable | Yes |
| Application | Fluorescence Microscopy |
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Technical Data for Sulfidefluor 7 AM
| M. Wt | 685.55 |
| Formula | C31H23N7O12 |
| Storage | Store at -20°C |
| CAS Number | 1416872-50-8 |
| PubChem ID | 89306941 |
| InChI Key | QIAZHSBWGCYJGQ-UHFFFAOYSA-N |
| Smiles | O=C(O1)C2=C(C=CC(C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=O)=C2)C31C4=CC=C(N=[N+]=[N-])C=C4OC5=C3C=CC(N=[N+]=[N-])=C5 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Sulfidefluor 7 AM
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 13.71 | 20 |
Preparing Stock Solutions for Sulfidefluor 7 AM
The following data is based on the product molecular weight 685.55. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 7.29 mL | 36.47 mL | 72.93 mL |
| 1 mM | 1.46 mL | 7.29 mL | 14.59 mL |
| 2 mM | 0.73 mL | 3.65 mL | 7.29 mL |
| 10 mM | 0.15 mL | 0.73 mL | 1.46 mL |
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Product Datasheets for Sulfidefluor 7 AM
References for Sulfidefluor 7 AM
References are publications that support the biological activity of the product.
Lin et al (2013) Cell-trappable fluorescent probes for endogenous hydrogen sulfide signaling and imaging H2O2-dependent H2S production. Proc.Natl.Acad.Sci.USA. 110 7131 PMID: 23589874
Lippert et al (2011) Reaction-based fluorescent probes for selective imaging of hydrogen sulfide in living cells. J.Am.Chem.Soc. 133 10078 PMID: 21671682
If you know of a relevant reference for Sulfidefluor 7 AM, please let us know.
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Keywords: Sulfidefluor 7 AM, Sulfidefluor 7 AM supplier, hydrogen, sulphide, H2S, fluorescent, probe, Fluorescent, Cell, Indicators, and, Sensors, 4943, Tocris Bioscience
4 Citations for Sulfidefluor 7 AM
Citations are publications that use Tocris products. Selected citations for Sulfidefluor 7 AM include:
Margreet R et al (2020) Periprocedural Hydrogen Sulfide Therapy Improves Vascular Remodeling and Attenuates Vein Graft Disease. J Am Heart Assoc 9 e016391 PMID: 33146045
Cheng et al (2016) Restoration of Hydrogen Sulfide Production in Diabetic Mice Improves Reparative Function of Bone Marrow Cells. Circulation 134 1467 PMID: 27660293
Chhabra et al (2018) Glucose-6-phosphate dehydrogenase is critical for suppression of cardiac hypertrophy by H2S. Cell Death Discov 4 6 PMID: 29531803
Xiaofeng et al (2021) Intracellular H2S production is an autophagy-dependent adaptive response to DNA damage. Cell Chem Biol 28 1669-1678.e5 PMID: 34166610
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Fluorescent Dyes and Probes Research Product Guide
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