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Submit ReviewTaxol Janelia Fluor® 549
Description: Yellow-fluorescent taxol derivative. Used for: microtubule staining. Application: flow cytometry, confocal microscopy, super resolution microscopy
Alternative Names: Taxol-JF549
Chemical Name: 2-(3-(Azetidin-1-ium-1-ylidene)-6-(azetidin-1-yl)-3H-xanthen-9-yl)-4-(((2S)-1-(((2aR,4S,4aS,6R,9S,11S,12S,12bS)-6,12b-diacetoxy-9-(((2R,3S)-3-benzamido-2-hydroxy-3-phenylpropanoyl)oxy)-12-(benzoyloxy)-11-hydroxy-4a,8,13,13-tetramethyl-5-oxo-2a,3,4,4a,5,6,9,10,11,12,12a,12b-dodecahydro-1H-7,11-methanocyclodeca[3,4]benzo[1,2-b]oxet-4-yl)oxy)-1-oxopropan-2-yl)carbamoyl)benzoate formate
Purity: ≥90% (HPLC)
Biological Activity for Taxol Janelia Fluor® 549
Key information: Taxol Janelia Fluor® 549 is a yellow-fluorescent taxol derivative for imaging of the microtubule cytoskeleton.
Used for: microtubule cytoskeleton imaging.
Application: flow cytometry, confocal microscopy, super resolution microscopy (SRM).
Properties and Photophysical Data: Excitation and emission maxima (λ) are 556 nm and 575 nm, respectively.
For more information on how Taxol-Janelia Fluor® 549 may be used, see our protocol.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Taxol Janelia Fluor® 549
| Emission Color | Yellow |
|---|---|
| Brightness | |
| λabs | 556 nm |
| λem | 575 nm |
| Application | Flow Cytometry, Confocal Microscopy, Super Resolution Microscopy (SRM) |
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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for Taxol Janelia Fluor® 549
| M. Wt | 1407.49 |
| Formula | C77H76N4O19.HCOOH |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| InChI Key | ZEBDFYUXCMYUMD-FZIPNWKRSA-N |
| Smiles | O=C(O[C@]12C([C@@H]([C@]3(C(C)(C([C@H](C4=O)OC(C)=O)=C([C@H](C3)OC([C@@H]([C@@H](NC(C5=CC=CC=C5)=O)C6=CC=CC=C6)O)=O)C)C)O)OC(C7=CC=CC=C7)=O)[C@]4([C@H](C[C@H]1OC2)OC([C@@H](NC(C8=CC=C(C([O-])=O)C(C9=C(C(OC%10=C9C=CC(N%11CCC%11)=C%10)=C/%12)C=CC%12=[N+]%13CCC\%13)=C8)=O)C)=O)C)C.[H]C(O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Taxol Janelia Fluor® 549
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 14.07 | 10 |
Preparing Stock Solutions for Taxol Janelia Fluor® 549
The following data is based on the product molecular weight 1407.49. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 7.1 mL | 35.52 mL | 71.05 mL |
| 0.5 mM | 1.42 mL | 7.1 mL | 14.21 mL |
| 1 mM | 0.71 mL | 3.55 mL | 7.1 mL |
| 5 mM | 0.14 mL | 0.71 mL | 1.42 mL |
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Product Datasheets for Taxol Janelia Fluor® 549
References for Taxol Janelia Fluor® 549
References are publications that support the biological activity of the product.
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Citations for Taxol Janelia Fluor® 549
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Protocols for Taxol Janelia Fluor® 549
The following protocol features additional information for the use of Taxol Janelia Fluor® 549 (Cat. No. 6267).
Literature in this Area
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Fluorescent Dyes and Probes Research Product Guide
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