Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewTBI
Description: Fluorogenic ligand for Broccoli RNA aptamer
Chemical Name: (Z)-3-((1H-Benzo[d]imidazol-4-yl)methyl)-5-(3,5-difluoro-4-hydroxybenzylidene)-2-thioxoimidazolidin-4-one
Purity: ≥98% (HPLC)
Biological Activity for TBI
TBI is a DFHBI derivative for imaging of single Broccoli-tagged mRNA in cells. Excitation and emission maxima (λ) when bound to Broccoli are 485 and 527 nm, respectively. TBI shows higher affinity for Broccoli aptamers compared with DFHBI-1T (Cat. No. 5610) (KD values are 71 and 305 nM, respectively). Extinction coefficient = 35,100 M-1 cm-1.
Licensing Information
Sold under license from Lucerna
Optical Data for TBI
| Emission Color | Green |
|---|---|
| λabs | 485 nm |
| λem | 527 nm |
| Extinction Coefficient (ε) | 35100 M-1cm-1 |
| Quantum Yield (φ) | 0.64 |
| Application | Live-cell Imaging, Fluorescence Microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for TBI
| M. Wt | 386.38 |
| Formula | C18H12F2N4O2S |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 2863639-29-4 |
| InChI Key | FFVXYOZVQLWDIF-NSIKDUERSA-N |
| Smiles | FC1=C(C(F)=CC(/C=C2NC(N(C\2=O)CC3=C4N=CNC4=CC=C3)=S)=C1)O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for TBI
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 7.73 | 20 |
Preparing Stock Solutions for TBI
The following data is based on the product molecular weight 386.38. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 12.94 mL | 64.7 mL | 129.41 mL |
| 1 mM | 2.59 mL | 12.94 mL | 25.88 mL |
| 2 mM | 1.29 mL | 6.47 mL | 12.94 mL |
| 10 mM | 0.26 mL | 1.29 mL | 2.59 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
Product Datasheets for TBI
References for TBI
References are publications that support the biological activity of the product.
Li et al (2021) Engineering fluorophore recycling in a fluorogenic RNA aptamer. Angew.Chem.Int.Ed.Engl. 60 24153 PMID: 34490956
If you know of a relevant reference for TBI, please let us know.
View Related Products by Target
Keywords: TBI, TBI supplier, fluorescence, fluorophores, fluorophore, single, RNA, imaging, fluorescent, protein, Broccoli, aptamers, Aptamer-based, Imaging, 7660, Tocris Bioscience
Citations for TBI
Citations are publications that use Tocris products.
Currently there are no citations for TBI. Do you know of a great paper that uses TBI from Tocris? Please let us know.
Reviews for TBI
There are currently no reviews for this product. Be the first to review TBI and earn rewards!
Have you used TBI?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals