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Description: Orange fluorescent dye; amine reactive
Chemical Name: 6-[2-Carboxy-4(5)-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-1,2,10,11-tetrahydro-2,2,10,10-tetramethyl-4,8-bis(sulfomethyl)pyrano[3,2-g:5,6-g']diquinolin-13-ium inner salt
Biological Activity for TFAX 568, SE
TFAX 568, SE is an amine reactive orange fluorescent dye. Insensitive to pH in the range 4 - 10. Forms bright and photostable conjugates with proteins and antibodies. Suitable for use in flow cytometry, 2-photon excitation microscopy, and super resolution mircoscopy techniques, such as dSTORM. Excitation maximum = 578 nm; emission maximum = 602 nm; quantum yield = 0.69; extinction coefficient = 60720 M-1cm-1.
Optical Data for TFAX 568, SE
| Emission Color | Orange |
|---|---|
| Brightness | |
| λabs | 578 nm |
| λem | 602 nm |
| Extinction Coefficient (ε) | 88,000 M-1cm-1 |
| Quantum Yield (φ) | 0.69 |
| Closest Laser line | 568 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Alternative For | Alexa Fluor® 568, Rhodamine red |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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Technical Data for TFAX 568, SE
| M. Wt | 791.8 |
| Formula | C37H33N3O13S2 |
| Storage | Store at -20°C |
| CAS Number | 878549-44-1 |
| PubChem ID | 25164098 |
| InChI Key | DJLBKHHGFYXTRY-UHFFFAOYSA-N |
| Smiles | CC(C=C1CS(=O)(O)=O)(C)NC2=C1C=C(C(C3=CC=C(C(ON4C(CCC4=O)=O)=O)C=C3C(O)=O)=C5C(O6)=CC7=[NH+]C(C)(C)C=C(CS(=O)([O-])=O)C7=C5)C6=C2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for TFAX 568, SE
References for TFAX 568, SE
References are publications that support the biological activity of the product.
Panchuk-Voloshina et al (1999) Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J.Histochem.Cytochem. 47 1179 PMID: 10449539
Van de Linde et al (2011) Direct stochastic optical reconstruction microscopy with standard fluorescent probes Nature Protocols 6 991
Rossi et al (2012) Super-resolution imaging of aquaporin-4 orthogonal arrays of particles in cell membranes. J.Cell Sci. 125 4405 PMID: 22718347
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
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