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Description: Cell-permeable fluorescent amyloid stain. Used for: in vitro Aβ staining in brain tissues.
Chemical Name: 2-[4-(Dimethylamino)phenyl]-3,6-dimethylbenzothiazolium chloride
Purity: ≥95% (HPLC)
Biological Activity for Thioflavin T
Key information: Thioflavin T is a cell-permeable fluorescent amyloid stain.
Used for: staining of insoluble senile plaques of Aβ in brain tissues, monitoring the kinetics of in vitro polyglutamine amyloid formation of tNhtt-42Q aggregates in a Huntington's disease cell model. Thioflavin T is used to confirm formation of β sheet structure from mutant huntingtin exon-1 aggregates (Ex1Q48) in vitro.
Application: fluorescence microscopy.
Properties and Photophysical Data: Thioflavin T is a cationic benzothiazole. It increases in fluorescence upon strong binding (Ki = 580 nM) to the stacked β sheets of amyloid fibrils. Excitation and emission maxima (λ) are 440 nm and 485 nm, respectively; quantum yield = 0.43; extinction coefficient = 31600 M-1cm-1.
This product is supplied with a high degree of hydration and some residual inorganic salts, the amount of which are batch dependent. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.
Optical Data for Thioflavin T
| Emission Color | Blue |
|---|---|
| λabs | 440 nm |
| λem | 485 nm |
| Extinction Coefficient (ε) | 31600 M-1cm-1 |
| Quantum Yield (φ) | 0.43 |
| Cell Permeable | Yes |
| Application | Amyloid Stain |
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Technical Data for Thioflavin T
| M. Wt | 318.86 |
| Formula | C17H19ClN2S |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 2390-54-7 |
| PubChem ID | 16953 |
| InChI Key | JADVWWSKYZXRGX-UHFFFAOYSA-M |
| Smiles | CC1=CC2=C([N+](C)=C(C3=CC=C(N(C)C)C=C3)S2)C=C1.[Cl-] |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Thioflavin T
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 1.59 | 5 | |
| water | 3.19 | 10 |
Preparing Stock Solutions for Thioflavin T
The following data is based on the product molecular weight 318.86. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 31.36 mL | 156.81 mL | 313.62 mL |
| 0.5 mM | 6.27 mL | 31.36 mL | 62.72 mL |
| 1 mM | 3.14 mL | 15.68 mL | 31.36 mL |
| 5 mM | 0.63 mL | 3.14 mL | 6.27 mL |
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Product Datasheets for Thioflavin T
References for Thioflavin T
References are publications that support the biological activity of the product.
Doi et al (2008) RNA-binding protein TLS is a major nuclear aggregate-interacting protein in huntingtin exon 1 with expanded polyglutamine-expressing cells. J.Biol.Chem. 283 6489 PMID: 18167354
Lee et al (2019) Fluorescence chemicals to detect insoluble and soluble amyloid-β aggregates. ACS Chem.Neurosci. 10 2647 PMID: 31009195
Schindler (2021) Small, seeding-competent huntingtin fibrils are prominent aggregate species in brains of zQ175 Huntington's disease knock-in mice. Front.Neurosci. 15 682172 PMID: 34239412
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals