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Description: Tissue clearing reagent kit
Biological Activity for Tissue Clearing Pro
Tissue Clearing Pro is a rapid, easy-to-use, reversible and non-destructive clearing and staining kit, which allows effective tissue clearing and staining of whole tissues. Tissue Clearing Pro is a solvent based tissue clearing technique, comparable to BABB and DISCO. Tissue Clearing Pro-Organoid does not cause significant shrinkage or tissue damage and does not use as harsh a solvent as other solvent based techniques.
This kit can clear a whole mouse brain of up to 8 mm thickness in 24 hours; for sections with a thickness of 1 mm this can be achieved in 2 hours. Tissue Clearing Pro is compatible with fluorescent protein, immunolabeling and small molecule staining techniques, as well as being suitable for use with all standard dyes and buffers. Following 3D imaging, Tissue Clearing Pro can be reversed for follow-up 2D histology staining. The kit comes with a getting started guide and a more comprehensive tissue clearing guidebook, providing detailed protocols. The Tissue Clearing Pro kit contains 30 mL of Tissue Clearing Pro clearing reagent 1 and 2, plus the 5 buffer solutions required to process tissues for immunolabeling, and is enough to process four whole mouse brains (approx. 2 g of tissue).
Please read the Tissue Clearing Pro Guidebook for more information and detailed protocols for using this kit.
Technical Data for Tissue Clearing Pro
| Storage | Store at RT |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Tissue Clearing Pro
References for Tissue Clearing Pro
References are publications that support the biological activity of the product.
Sargent (2019) Clarification and confocal imaging of the nonhuman primate placental micro-anatomy. Biotechniques 66 73 PMID: 30370778
Regla-Nava et al (2019) Detection of Zika virus in mouse mammary gland and breast milk. PLoS Negl.Trop.Dis. 13 e0007080 PMID: 30742628
Robino et al (2020) Spatial and biochemical interactions between bone marrow adipose tissue and hematopoietic stem and progenitor cells in rhesus macaques. Bone 133 115248 PMID: 31972314
Choi et al (2020) Innate lymphoid cells play a pathogenic role in pericarditis. Cell Rep. 30 2989 PMID: 32130902
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Citations for Tissue Clearing Pro
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals