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Acridonylalanine

Cat. No. 8086 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： Fluorescent unnatural amino acid.
化学名： Sodium 2-Amino-3-(9-oxo-9,10-dihydroacridin-2-yl)propanoate
纯度： ≥95% (HPLC)
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生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for Acridonylalanine

Key information: Acridonylalanine is a fluorescent unnatural amino acid. Can be used to specifically label a protein of interest during protein translation. Suitable for lifetime and fluorescence resonance energy transfer (FRET) studies.

Used for: labeling proteins, monitoring protein interactions and conformational changes through fluorescence polarization or lifetime experiments.

Application: fluorescence lifetime imaging microscopy (FLIM), FRET interactions with common fluorophores such as methoxycoumarin.

Properties and Photophysical Data: highly photostable, high quantum yield and long fluorescence lifetime in water. Excitation and emission maxima (λ) are 385 nm and 420 nm, respectively.

Optical Data for Acridonylalanine

λabs 385 nm
λem 420 nm

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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技术数据 for Acridonylalanine

分子量 304.28
公式 C16H13N2NaO3
储存 Store at -20°C
纯度 ≥95% (HPLC)
PubChem ID 496123694
InChI Key XZLOZGUCQWEABV-UHFFFAOYSA-M
Smiles O=C1C2=CC(CC(C([O-])=O)N)=CC=C2NC3=CC=CC=C31.[Na+]

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Acridonylalanine

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 3.04 10
water 3.04 10

制备储备液 for Acridonylalanine

以下数据基于产品分子量 304.28。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
0.1 mM 32.86 mL 164.32 mL 328.64 mL
0.5 mM 6.57 mL 32.86 mL 65.73 mL
1 mM 3.29 mL 16.43 mL 32.86 mL
5 mM 0.66 mL 3.29 mL 6.57 mL

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产品说明书 for Acridonylalanine

分析证书/产品说明书
化学品安全技术说明书

参考文献 for Acridonylalanine

参考文献是支持产品生物活性的出版物。

Hostetler et al (2018) Systematic evaluation of soluble protein expression using a fluorescent unnatural amino acid reveals no reliable predictors of tolerability. ACS Chem.Biol. 13 2855 PMID: 30216041

Speight et al (2013) Efficient synthesis and in vivo incorporation of acridon-2-ylalanine, a fluorescent amino acid for lifetime and Förster resonance energy transfer/luminescence resonance energy transfer studies. J.Am.Chem.Soc. 135 18806 PMID: 24303933

If you know of a relevant reference for Acridonylalanine, please let us know.

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Fluorescent Dyes and Probes Research Product Guide

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