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Submit ReviewBafilomycin A1
说明： H+-ATPase (vacuolar) inhibitor
化学名： (3Z,5E,7R,8S,9S,11E,13E,15S,16R)-8-Hydroxy-16-[(1S,2R,3S)-2-hydroxy-1-methyl-3-[(2R,4R,5S,6R)-tetrahydro-2,4-dihydroxy-5-methyl-6-(1-methylethyl)-2H-pyran-2-yl]butyl]-3,15-dimethoxy-5,7,9,11-tetramethyloxacyclohexadeca-3,5,11,13-tetraen-2-one
纯度： ≥95% (HPLC)
生物活性 for Bafilomycin A1
Bafilomycin A1 is a highly potent, selective inhibitor of vacuolar H+-ATPases (IC50 = 0.6 - 1.5 nM in bovine chromaffin granules). Selective for v-ATPase over other ATP hydrolyzing enzymes such as F-ATPases and the H+/K+-ATPase (P-ATPase). Thought to inhibit autophagy either by blocking autophagosome-lysosome fusion (in H4IIE cells), or by blocking lysosomal degradation. Bafilomycin A1 accelerates wound healing in db/db mice. Low dose Bafilomycin A1 attenuates patient-derived CD34+CD19+ leukemia stem cells (LSC) and inhibits LSC proliferation in an animal model. Bafilomycin A1 impairs Zika virus infection in vitro.
技术数据 for Bafilomycin A1
| 分子量 | 622.84 |
| 公式 | C35H58O9 |
| 储存 | Store at -20°C |
| 纯度 | ≥95% (HPLC) |
| CAS Number | 88899-55-2 |
| PubChem ID | 72311 |
| InChI Key | WEEFNMFMNMASJY-UHFFFAOYSA-M |
| Smiles | O[C@H]([C@H](C)/C=C(C)/C=C1OC)[C@@H](C)C/C(C)=C/C=C\[C@H](OC)C([C@@H](C)[C@H]([C@@H]([C@@]2(O)C[C@@H](O)[C@H](C)[C@@H]([C@H](C)C)O2)C)O)OC/1=O |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for Bafilomycin A1
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 4.98 | 8 |
制备储备液 for Bafilomycin A1
以下数据基于产品分子量 622.84。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.08 mM | 20.07 mL | 100.35 mL | 200.69 mL |
| 0.4 mM | 4.01 mL | 20.07 mL | 40.14 mL |
| 0.8 mM | 2.01 mL | 10.03 mL | 20.07 mL |
| 4 mM | 0.4 mL | 2.01 mL | 4.01 mL |
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产品说明书 for Bafilomycin A1
参考文献 for Bafilomycin A1
参考文献是支持产品生物活性的出版物。
Gagliardi et al (1999) Chemistry and stucture activity relationships of bafilomycin A1, a potent and selective inhibitor of the vacuolar H+-ATPase. Curr.Med.Chem. 6 1197 PMID: 10519916
Yamamoto et al (1998) Bafilomycin A1 prevents maturation of autophagic vacuoles by inhibiting fusion between autophagosomes and lysosomes in rat hepatoma cell line, H-4-II-E cells. Cell Struct.Funct. 23 33 PMID: 9639028
Fass et al (2006) Microtubules support production of starvation-induced autophagosomes but not their targeting and fusion with lysosomes. J.Biol.Chem. 281 36303 PMID: 16963441
Mauthe et al (2018) Chloroquine inhibits autophagic flux by decreasing autophagosome-lysosome fusion. Autophagy 14 1435 PMID: 29940786
Wang et al (2020) Bafilomycin A1 accelerates chronic refractory wound healing in db/db mice. Biomed.Res.Int. 6265701 PMID: 32714982
Xu et al (2020) Bafilomycin A1 targets patient-derived CD34 + CD19 + leukemia stem cells. Haematologica 105 e17 PMID: 31097633
Sabino et al (2019) Bafilomycin A1 and U18666A efficiently impair ZIKV infection in vitro. Viruses 11 524 PMID: 31174294
If you know of a relevant reference for Bafilomycin A1, please let us know.
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关键词: Bafilomycin A1, Bafilomycin A1 supplier, H+-ATPase, vacuolar, inhibitors, inhibits, Hydrogen-ATPase, Ion, Transporters, Pumps, BafilomycinA1, antibiotics, Antibiotics, Autophagy, 1334, Tocris Bioscience
52 篇 Bafilomycin A1 的引用文献
引用文献是使用了 Tocris 产品的出版物。 Bafilomycin A1 的部分引用包括：
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test.
By
Xiaogang Wang on 06/24/2021
分析类型： In Vitro
种属： Human
细胞系/组织： Human Macrophages
We used BafA inhibitor to investigate the autophagy induced by bacterial toxins. To confirm whether BafA1 works, human macrophages were treated with or without 100 nM BafA for 1 h, and the levels of LC3-I and LC3-II within human macrophages were determined by western blot using LC3 antibody. As expected (shown in figure), treatment of cells with BafA1 resulted in an increased level of LC3-I and LC3-II, suggesting that the fusion of autophagosomes and lysosomes was blocked and resulted in the accumulation of autophagosomes.
BAF.
By
shubham dubey on 05/28/2021
分析类型： In Vitro
种属： Rat
细胞系/组织： H9C2
Treated H9C2 cells to check the autophagy.
Good autophagy inhibitor.
By
JING HE on 03/27/2020
分析类型： In Vitro
种属： Human
细胞系/组织： HT29
HT29 cells transfected with ad-mRFP-GFP-LC3 adenovirus after incubation with or without LPS and Baf A1 at different time points.
