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Biotinyl Tyramide

Cat. No. 6241 2 篇引用文献 2 条评论 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
Pricing Availability   Qty
说明： Reagent widely used for signal amplification in IHC and FISH
化学名： (3aS,4S,6aR)-Hexahydro-N-[2-(4-hydroxyphenyl)ethyl]-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide
纯度： ≥98% (HPLC)
说明书
引用文献 (2)
评论 (2)
生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for Biotinyl Tyramide

Biotinyl Tyramide is a reagent widely used for signal amplification in IHC and fluorescent in situ hybridization (ISH) and APEX proximity labeling. HRP catalyzes localized deposition of multiple biotinyl tyramide molecules (catalyzed reporter deposition, CARD), which can be detected using a labeled streptavidin conjugate. The localization of deposition around HRP leads to good resolution. Suitable for either fluorescence or chromogenic detection. Biotinyl Tyramide is used as a substrate for APEX proximity labeling.

技术数据 for Biotinyl Tyramide

分子量 363.47
公式 C18H25N3O3S
储存 Store at -20°C
纯度 ≥98% (HPLC)
CAS Number 41994-02-9
PubChem ID 21826423
InChI Key VZWXNOBHWODXCW-ZOBUZTSGSA-N
Smiles [H][C@]12CS[C@H]([C@]1(NC(N2)=O)[H])CCCCC(NCCC3=CC=C(C=C3)O)=O

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Biotinyl Tyramide

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 36.35 100

制备储备液 for Biotinyl Tyramide

以下数据基于产品分子量 363.47。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
1 mM 2.75 mL 13.76 mL 27.51 mL
5 mM 0.55 mL 2.75 mL 5.5 mL
10 mM 0.28 mL 1.38 mL 2.75 mL
50 mM 0.06 mL 0.28 mL 0.55 mL

Molarity Calculator

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产品说明书 for Biotinyl Tyramide

分析证书/产品说明书
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化学品安全技术说明书

参考文献 for Biotinyl Tyramide

参考文献是支持产品生物活性的出版物。

Bobrow et al (1989) Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays. J.Immunol.Methods 125 279 PMID: 2558138

McKay et al (1997) Amplification of fluorescent in situ hybridisation signals in formalin fixed paraffin wax embedded sections of colon tumour using biotinylated tyramide. Mol.Pathol. 50 322 PMID: 9536283

If you know of a relevant reference for Biotinyl Tyramide, please let us know.

按标靶查看相关产品

关键词: Biotinyl Tyramide, Biotinyl Tyramide supplier, in, situ, hybridization, ISH, immunohistochemistry, IHC, fluorescence, fluorescent, tyramide, signal, amplification, APEX, proximity, labeling, Tyramide, Signaling, Amplification, (TSA), 6241, Tocris Bioscience

2 篇 Biotinyl Tyramide 的引用文献

引用文献是使用了 Tocris 产品的出版物。 Biotinyl Tyramide 的部分引用包括：

Jonas et al (2022) The Development of Hindlimb Postural Asymmetry Induced by Focal Traumatic Brain Injury Is Not Related to Serotonin 2A/C Receptor Expression in the Spinal Cord. Int J Mol Sci 23 PMID: 35628167

Nicholas T et al (2022) Proximity-based labeling reveals DNA damage-induced phosphorylation of fused in sarcoma (FUS) causes distinct changes in the FUS protein interactome. J Biol Chem 298 102135 PMID: 35709984

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Biotinyl Tyramide 的评论

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Biotinyl tyramide used successfully for BP-labelling of mitochondrial proteins.
By Anonymous on 02/12/2020
分析类型： In Vitro
种属： Human

BP-labelling experiment in SH-SY5Y cells stably expressing MTS-APEX2. Tested range 500-1000µM biotinyl tyramide.

review image
Great product for Biotinylation.
By Anonymous on 02/07/2020
分析类型： In Vitro
种属： Human
细胞系/组织： SH-SY5Y

We use biotinyl tyramide to biotinylate cells - we have done this successfully without any issues to date. Biotinylated proteins can be detected by western blot using a streptavidin conjugated antibody (see image)

review image