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CCCP

Cat. No. 0452 4 篇引用文献 1 条评论 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： Oxidative phosphorylation uncoupler
化学名： Carbonyl cyanide 3-chlorophenylhydrazone
纯度： ≥98% (HPLC)
说明书
引用文献 (4)
评论 (1)
文献 (1)
生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for CCCP

CCCP is a protonophore. It disrupts mitochondria membrane potential by transporting protons across the mitochondrial inner membrane, which interferes with the proton gradient and ATP synthesis. CCCP is a widely used as an uncoupler of oxidative phosphorylation to induce mitochondrial depolarization.

技术数据 for CCCP

分子量 204.62
公式 C9H5ClN4
储存 Store at RT
纯度 ≥98% (HPLC)
CAS Number 555-60-2
PubChem ID 2603
InChI Key UGTJLJZQQFGTJD-UHFFFAOYSA-N
Smiles ClC1=CC=CC(N/N=C(C#N)\C#N)=C1

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for CCCP

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 20.46 100

制备储备液 for CCCP

以下数据基于产品分子量 204.62。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
1 mM 4.89 mL 24.44 mL 48.87 mL
5 mM 0.98 mL 4.89 mL 9.77 mL
10 mM 0.49 mL 2.44 mL 4.89 mL
50 mM 0.1 mL 0.49 mL 0.98 mL

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产品说明书 for CCCP

分析证书/产品说明书
选择另一批次：
化学品安全技术说明书

参考文献 for CCCP

参考文献是支持产品生物活性的出版物。

Heytler and Pritchard (1962) A new class of uncoupling agents - carbonyl cyanide phenylhydrazones. Biochem.Biophys.Res.Commun. 7 272 PMID: 13907155

If you know of a relevant reference for CCCP, please let us know.

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关键词: CCCP, CCCP supplier, Oxidative, phosphorylation, uncoupler, protonophore, proton, gradient, mitochondrial, depolarization, Phosphorylation, 0452, Tocris Bioscience

4 篇 CCCP 的引用文献

引用文献是使用了 Tocris 产品的出版物。 CCCP 的部分引用包括：

Choubey et al (2014) BECN1 is involved in the initiation of mitophagy: it facilitates PARK2 translocation to mitochondria. Autophagy 10 1105 PMID: 24879156

Purnell (2014) Efavirenz induces neuronal autophagy and mitochondrial alterations. J Pharmacol Exp Ther 351 250 PMID: 25161171

Chouhan et al (2010) Presynaptic mitochondria in functionally different motor neurons exhibit similar affinities for Ca2+ but exert little influence as Ca2+ buffers at nerve firing rates in situ. J Neurosci 30 1869 PMID: 20130196

Guo et al (2015) Use of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (hiPSC-CMs) to Monitor Compound Effects on Cardiac Myocyte Signaling Pathways. Curr Protoc Chem Biol 7 141 PMID: 26331525

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CCCP 的评论

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Mitochondrial studies in live neurons.
By Karthik krishnamurthy on 10/24/2018
分析类型： In Vitro
种属： Rat
细胞系/组织： spinal cord neurons

TMRM fluorescence intensity before and after CCCP addition was used for the quantitation of change in mitochondrial membrane potential in spinal cord neurons induced by glutamate receptor agonist AMPA

Time lapse images of TMRM fluorescence of mitochondria rich regions in neurons was recorded. The fluorescence intensity (F) at a given time point was normalized to the initial fluorescence intensity (F0) before addition of the AMPA. At the end of each experiment CCCP (750 nM) was added to the bath. CCCP collapses the mitochondrial membrane potential and decreases the TMRM fluorescence.

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