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CELT-327

Cat. No. 7954 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： Potent and selective hA2B/A3 adenosine receptor fluorescent antagonist
纯度： ≥95% (HPLC)
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生物活性 for CELT-327

CELT-327 is a potent and selective fluorescent hA2B/A3 adenosine receptor antagonist (Ki values are 35.6 nM and 45.7 nM at A2B and A3 receptors respectively). It shows dose-dependent membrane fluorescent labeling in HCT116 cells expressing A2B adenosine receptors, with rapid diffusion and homogeneous distribution of the fluorescence signal within the cell monolayer. In live HCT116 compact spheroids, CELT-327 displays homogeneous staining across the entire spheroid diameter, reaching deep spheroid regions. CELT-327 can be used as a fluorescent adenosine receptor probe for fluorescence binding assays, live cell imaging, flow cytometry, and fluorescence polarization assays. It is a suitable replacement for GPCR radiolabeling ligands. Excitation and emission maxima (λ) are 589 nm and 616 nm, respectively. Wavelengths are compatible with use as an acceptor dye in TR-FRET assays, together with the CoraFluor™ 1 (Cat. No. 7920) TR-FRET donor.

Add 75 μL of assay buffer containing 1% of DMSO to obtain a 100 μM stock solution, and use 250 nM as a working concentration.

Scientific Data

Live-cell Imaging Application of CELT-327 to live colorectal cancer cells View Larger

Application of CELT-327 to live colorectal cancer cells. HeLa cells were co-cultured with fibroblasts from the stroma around tumors (CAFs) previously labeled in green with a cell-tracker. Both cell types were incubated with CELT-327 (250 nM). The figure shows in red the plasma membrane staining revealed by CELT-327 specifically in HeLa cells.

Fixed-cell Imaging Application of CELT-327 to fixed colorectal cancer cells. View Larger

Application of CELT-327 to fixed colorectal cancer cells. HCT116 cells were fixed with PFA 4% (20 mins at room temperature) and then incubated with CELT-327 (250 nM) for 30 mins at room temperature, washed and visualized immediately (A), or 96h post-staining (B).

许可信息

Sold under license from Celtarys Research

Optical Data for CELT-327

Emission Color Orange
λabs 589 nm
λem 616 nm
Closest Laser line 561 nm
Application Confocal microscopy, High-content screening

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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技术数据 for CELT-327

分子量 1334.19
储存 Store at -20°C
纯度 ≥95% (HPLC)

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

参考文献 for CELT-327

参考文献是支持产品生物活性的出版物。

Barbazán et al (2022) Identification of A2BAR as a potential target in colorectal cancer using novel fluorescent GPCR ligands. Biomed.Pharmacother. 153 113408 PMID: 36076535

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Enabling Research By Provision of Chemical Tools Poster

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The Tocris chemistry team has considerable chemistry knowledge and skill which it has recently been applying to the generation of new chemical probes from known tools and compounds to help answer biological questions. This poster presents some of the work carried out by Tocris scientists and focuses on two areas: the generation of libraries of chemical building blocks to support Degrader (PROTAC) research and the development of a fluorescent probe to study integrin biology. Presented at Chemical Tools for Complex Biological Systems II, 2019, Janelia Research Campus, USA.