Cyanine 3 Tyramide

Cat. No. 6457 提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

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说明： Orange fluorescent reagent widely used for signal amplification in IHC and FISH

化学名： 2-[3-[(1-Ethyl-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene)-1-propen-1-yl]-1-[6-[[2-(4-hydroxyphenyl)ethyl]amino-6-oxohexyl]-3,3-dimethyl-5-sulfo-3H-indolium inner salt

纯度： ≥95% (HPLC)

生物活性技术数据溶解性计算器说明书参考文献

生物活性 for Cyanine 3 Tyramide

Cyanine 3 Tyramide is an orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.

Optical Data for Cyanine 3 Tyramide

Emission Color	Orange
λ_abs	550 nm
λ_em	563 nm
Extinction Coefficient (ε)	150000 M^-1cm^-1
Quantum Yield (φ)	0.31
Closest Laser line	532 nm
Application	Tyramide Signal Amplification (TSA) in IHC, ICC, ISH, FISH

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

技术数据 for Cyanine 3 Tyramide

分子量	749.94
公式	C₃₉H₄₇N₃O₈S₂
储存	Store at -20°C
纯度	≥95% (HPLC)
CAS Number	174961-75-2
InChI Key	JPXIOSIRIWBDIC-UHFFFAOYSA-N
Smiles	CCN1C2=C(C=C(C=C2)S(O)(=O)=O)C(/C1=C\C=C\C3=[N+](CCCCCC(NCCC4=CC=C(O)C=C4)=O)C5=C(C=C(C=C5)S([O-])(=O)=O)C3(C)C)(C)C

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Cyanine 3 Tyramide

	溶剂	最高浓度 mg/mL	最高浓度 mM
溶解性
溶解性	DMSO	12	16

制备储备液 for Cyanine 3 Tyramide

以下数据基于产品分子量 749.94。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
0.16 mM	8.33 mL	41.67 mL	83.34 mL
0.8 mM	1.67 mL	8.33 mL	16.67 mL
1.6 mM	0.83 mL	4.17 mL	8.33 mL
8 mM	0.17 mL	0.83 mL	1.67 mL

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产品说明书 for Cyanine 3 Tyramide

分析证书/产品说明书

化学品安全技术说明书

参考文献 for Cyanine 3 Tyramide

参考文献是支持产品生物活性的出版物。

Schmidt et al (1997) Signal amplification in the detection of single-copy DNA and RNA by enzyme-catalyzed deposition (CARD) of the novel fluorescent reporter substrate Cy3.29-tyramide. J.Histochem.Cytochem. 45 365 PMID: 9071318

van Gijlswijk et al (1997) Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J.Histochem.Cytochem. 45 375 PMID: 9071319

Hopman et al (1998) Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for in situ hybridization using CARD amplification. J.Histochem.Cytochem. 46 771 PMID: 9603790

Schriml et al (1999) Tyramide signal amplification (TSA)-FISH applied to mapping PCR-labeled probes less than 1 kb in size. Biotechniques 27 608 PMID: 10489619

Zhao et al (2013) Characterization of GABAergic neurons in the mouse lateral septum: a double fluorescence in situ hybridization and immunohistochemical study using tyramide signal amplification. PLoS One 8 e73750 PMID: 23967349

If you know of a relevant reference for Cyanine 3 Tyramide, please let us know.

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