Cyanine 5 Tyramide

Cat. No. 6458 提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

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说明： Red fluorescent reagent widely used for signal amplification in IHC and FISH

别名： Cy5 Tyramide

化学名： 2-[5-[1,3-Dihydro-1-[6-[[2-(4-hydroxyphenyl)ethyl]amino]-6-oxohexyl]3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-3H-indolium inner salt

纯度： ≥95% (HPLC)

生物活性技术数据溶解性计算器说明书参考文献

生物活性 for Cyanine 5 Tyramide

Cyanine 5 Tyramide is a red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.

Optical Data for Cyanine 5 Tyramide

Emission Color	Red
λ_abs	651 nm
λ_em	665 nm
Extinction Coefficient (ε)	250000 M^-1cm^-1
Closest Laser line	647 nm
Application	Tyramide Signal Amplification (TSA) in IHC, ICC, ISH, FISH

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

技术数据 for Cyanine 5 Tyramide

分子量	775.97
公式	C₄₁H₄₉N₃O₈S₂
储存	Store at -20°C
纯度	≥95% (HPLC)
CAS Number	1431148-26-3
PubChem ID	135290897
InChI Key	NHSJCIBSNKLAMA-UHFFFAOYSA-N
Smiles	CC[N+]1=C(C(C)(C2=CC(S([O-])(=O)=O)=CC=C12)C)/C=C/C=C/C=C3N(C4=CC=C(S(O)(=O)=O)C=C4C/3(C)C)CCCCCC(NCCC5=CC=C(C=C5)O)=O

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Cyanine 5 Tyramide

	溶剂	最高浓度 mg/mL	最高浓度 mM
溶解性
溶解性	DMSO	11.64	15

制备储备液 for Cyanine 5 Tyramide

以下数据基于产品分子量 775.97。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
0.15 mM	8.59 mL	42.96 mL	85.91 mL
0.75 mM	1.72 mL	8.59 mL	17.18 mL
1.5 mM	0.86 mL	4.3 mL	8.59 mL
7.5 mM	0.17 mL	0.86 mL	1.72 mL

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产品说明书 for Cyanine 5 Tyramide

分析证书/产品说明书

化学品安全技术说明书

参考文献 for Cyanine 5 Tyramide

参考文献是支持产品生物活性的出版物。

Chanda et al (2017) Identification of the SUMO E3 ligase PIAS1 as a potential survival biomarker in breast cancer. PLoS One 12 e0177639 PMID: 28493978

Matsumoto et al (2017) Aldehyde dehydrogenase 1B1: a novel immunohistological marker for colorectal cancer. Br.J.Cancer 117 1537 PMID: 28881356

Wong et al (2001) Identification of differentially expressed genes from ovarian cancer cells by MICROMAX cDNA microarray system. Biotechniques 30 670 PMID: 11252802

If you know of a relevant reference for Cyanine 5 Tyramide, please let us know.

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