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DAPI

Cat. No. 5748 14 篇引用文献 2 条评论 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： Blue-fluorescent DNA stain / dye. Used for: nuclear counterstain, fixed and live-cell staining, assessing apoptosis. Application: flow cytometry, confocal microscopy, immunofluorescence (IHC, ICC), ISH
别名： DAPI Dye
化学名： 2-[4-(Aminoiminomethyl)phenyl]-1H-Indole-6-carboximidamide hydrochloride
纯度： ≥95% (HPLC)
说明书
引用文献 (14)
评论 (2)
文献 (1)
生物活性 Scientific Data 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for DAPI

Key information: DAPI is a widely used blue-fluorescent DNA stain / dye. Membrane impermeant to live cells. For nuclear counterstain, fixed and live-cell staining (at high concentration).

Used for: nuclear counterstain, fixed and live-cell staining (at high concentration), DNA visualization, chromosome staining, DNA staining in agarose gels, apoptosis analysis, mouse embryos or fetal organs visualization.

Application: flow cytometry, confocal microscopy, immunofluorescence (IHC, ICC), and ISH.

Properties and Photophysical Data: DAPI stain binds to AT-rich regions of DNA and fluorescence increases by approximately 20-fold when bound to double-stranded DNA. It is reported to be more photostable than Hoechst 33342 (Cat. No. 5117). Suitable for multicolor fluorescence microscopy experiments. Excitation and emission maxima (λ) are 350 nm and 470 nm, respectively.

Scientific Data

Confocal Microscopy DAPI staining View Larger

DAPI staining. HeLa cells were fixed for 10 min with 4% paraformaldehyde and permeabilized. DAPI was added at 0.1 μg/mL and stained cells were imaged with an LSM880 confocal microscope (Obj. 63X/1.4 oil). Scale bar = 10 μm.

Confocal Microscopy DAPI counterstaining alongside MitoBrilliant™ Live 646 View Larger

DAPI counterstaining alongside MitoBrilliant™ Live 646. HeLa cells incubated with MitoBrilliant™ Live 646 (Catalog # 7417), 100 nM for 40 min. Cells were counterstained with DAPI. Image taken using an LSM880 Confocal using a 63X objective. Scale bar = 10 μm.

Fluorescence Imaging RNAScope™ Assay with DAPI Counterstain View Larger

RNAScope™ Assay with DAPI Counterstain. 3-plex RNAScope™ Multiplex Fluorescent v2 Assay on HeLa cells with TSA Vivid™ dyes 520, 570 and 650 (Catalog # 7523, 7526 and 7527, Tocris). Nuclei are counter-stained with DAPI stain (blue). All dyes were used at 1:1500 dilution. Markers shown are Polr2a (green), PPIB (red) and UBC (white).

Optical Data for DAPI

DAPI Dye Spectra
Emission Color Blue
λabs 350 nm
λem 470 nm
Closest Laser line 405 nm
Application Flow Cytometry, ISH, Confocal Microscopy, Immunofluorescence (IHC, ICC)

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

技术数据 for DAPI

分子量 350.25
公式 C16H15N5.2HCl
储存 Store at -20°C
纯度 ≥95% (HPLC)
CAS Number 28718-90-3
PubChem ID 160166
InChI Key FPNZBYLXNYPRLR-UHFFFAOYSA-N
Smiles N=C(N)C(C=C1)=CC=C1C2=CC3=CC=C(C(N)=N)C=C3N2.Cl.Cl

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for DAPI

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 35.02 100
water 3.5 10 温和加热

制备储备液 for DAPI

以下数据基于产品分子量 350.25。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
1 mM 2.86 mL 14.28 mL 28.55 mL
5 mM 0.57 mL 2.86 mL 5.71 mL
10 mM 0.29 mL 1.43 mL 2.86 mL
50 mM 0.06 mL 0.29 mL 0.57 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Dilution Calculator

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产品说明书 for DAPI

分析证书/产品说明书
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化学品安全技术说明书

参考文献 for DAPI

参考文献是支持产品生物活性的出版物。

Russell et al (1975) A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature 253 461 PMID: 46112

Mildner et al (1978) Interaction of 4'-6-diamidino-2-phenylindole to nucleic acids, and its implication to their template activity in RNA-polymerase reaction of E. coli bacteria and of Friend-virus infected mouse spleen. Cancer Lett. 4 89 PMID: 348301

Chazotte (2011) Labeling nuclear DNA using DAPI. Cold Spring Harb.Protoc. 1 prot5556 PMID: 21205856

Kapuscinski (1995) DAPI: a DNA-specific fluorescent probe. Biotech.Histochem. 70 220 PMID: 858020

Sandell et al (2018) DAPI staining of whole-mount mouse embryos or fetal organs. Cold Spring Harb.Protoc. 10 prot094029 PMID: 30275072

If you know of a relevant reference for DAPI, please let us know.

按标靶查看相关产品

关键词: DAPI, DAPI supplier, fluorescent, DNA, stains, cell, permeable, dapi, dyes, apoptosis, staining, nuclear, counterstain, flow, cytometry, DAPI, Dye, Fluorescent, Stains, Flow, Cytometry, Counterstains, 5748, Tocris Bioscience

14 篇 DAPI 的引用文献

引用文献是使用了 Tocris 产品的出版物。 DAPI 的部分引用包括：

Elizabeth et al (2021) A protocol for rapid pericyte differentiation of human induced pluripotent stem cells. STAR Protoc 2 100261 PMID: 33490977

Daniel F et al (2021) Granzyme A Produced by γ9δ2 T Cells Activates ER Stress Responses and ATP Production, and Protects Against Intracellular Mycobacterial Replication Independent of Enzymatic Activity. Front Immunol 12 712678 PMID: 34413857

Digvijay et al (2021) HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells. Science 371 PMID: 33335017

Thanaphum et al (2020) NOTCH2 participates in Jagged1-induced osteogenic differentiation in human periodontal ligament cells. Sci Rep 10 13329 PMID: 32770090

Dean P et al (2020) Gut-Resident Lactobacilli Activate Hepatic Nrf2 and Protect Against Oxidative Liver Injury. Cell Metab 31 956-968.e5 PMID: 32213347

Allen et al (2020) Indirect regulation of HMGB1 release by gasdermin D. Nat Commun 11 4561 PMID: 32917873

Thanaphum et al (2023) Intermittent compressive force regulates dentin matrix protein 1 expression in human periodontal ligament stem cells. J Dent Sci 18 105-111 PMID: 36643268

Yelyzaveta et al (2023) BdLT-Seq as a barcode decay-based method to unravel lineage-linked transcriptome plasticity. Nat Commun 14 1085 PMID: 36841849

Yu et al (2023) Gut Microbiota-Derived Glutamine Attenuates Liver Ischemia/Reperfusion Injury via Macrophage Metabolic Reprogramming. Cell Mol Gastroenterol Hepatol 15 1255-1275 PMID: 36706918

Paul L et al (2023) Regulation of Cx43 Gap Junction Intercellular Communication by Bruton's Tyrosine Kinase and Interleukin-2-Inducible T-Cell Kinase. Biomolecules 13 PMID: 37189407

Satu et al (2022) Interplay of vascular endothelial growth factor receptors in organ-specific vessel maintenance. J Exp Med 219 PMID: 35050301

Titikorn et al (2022) Modulation of mitochondrial dynamics rescues cognitive function in rats with 'doxorubicin-induced chemobrain' via mitigation of mitochondrial dysfunction and neuroinflammation. FEBS J 289 6435-6455 PMID: 35514149

Marit H et al (2022) A mesenchymal to epithelial switch in Fgf10 expression specifies an evolutionary-conserved population of ionocytes in salivary glands. Cell Rep 39 110663 PMID: 35417692

Nipon et al (2022) Erythropoietin administration exerted neuroprotective effects against cardiac ischemia/reperfusion injury. Curr Res Pharmacol Drug Discov 3 100124 PMID: 36568264

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DAPI 的评论

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DAPI staining of PC3 cells to detect apoptosis.
By Anonymous on 12/13/2018
种属： Human

DAPI staining of PC3 cells treated with DR5 monoclonal antibodies to detect apoptosis and observing it with Floid Fluorescence Microscopy System.

review image
Excellent counter-stain....
By Purnima Gupta on 01/10/2018
分析类型： In Vivo
种属： Mouse
细胞系/组织： Brain

Used as counter stain in IHC

该领域的文献

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals