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dTAG-47

Cat. No. 7530 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： Degrades mutant FKBP12F36V fusion proteins; useful alternative to genetic methods for target validation
化学名： (1R)-3-(3,4-Dimethoxyphenyl)-1-(2-(2-((8-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-5-yl)amino)octyl)amino)-2-oxoethoxy)phenyl)propyl (2S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carboxylate
纯度： ≥97% (HPLC)
说明书
引用文献
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文献 (3)
生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for dTAG-47

dTAG-47 is a degrader targeting mutant FKBP12F36V fusion proteins. Comprises a ligand selective for F36V single-point mutated FKBP12, a linker and a cereblon-binding ligand. Application of dTAG-47 induces rapid, reversible and selective degradation of FKBP12F36V fusion proteins in vitro and in vivo. dTAG is generalizable to a range of fusion proteins; useful as an alternative to genetic methods for target validation. See also dTAG-13 and dTAG-7.

Negative control (Cat. No. 7531) also available.

FKBP12F36V can be expressed as a fusion with a target protein of interest using genome engineering techniques, via transgene expression or CRISPR-mediated locus-specific knock-in.

Plasmid vectors for the lentiviral expression and CRISPR-mediated knock-in of FKBP12F36V are available from Addgene.

许可信息

Sold under license from Dana-Farber Cancer Institute

技术数据 for dTAG-47

分子量 1076.25
公式 C59H73N5O14
储存 Store at -20°C
纯度 ≥97% (HPLC)
CAS Number 2265886-81-3
PubChem ID 153280948
InChI Key OQXAQHQPOQLCAE-ODPQSFPTSA-N
Smiles CC[C@@H](C1=CC(OC)=C(C(OC)=C1)OC)C(N2CCCC[C@H]2C(O[C@@H](C3=C(C=CC=C3)OCC(NCCCCCCCCNC4=CC=C5C(N(C(C5=C4)=O)C6CCC(NC6=O)=O)=O)=O)CCC7=CC(OC)=C(C=C7)OC)=O)=O

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for dTAG-47

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 107.62 100

制备储备液 for dTAG-47

以下数据基于产品分子量 1076.25。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
1 mM 0.93 mL 4.65 mL 9.29 mL
5 mM 0.19 mL 0.93 mL 1.86 mL
10 mM 0.09 mL 0.46 mL 0.93 mL
50 mM 0.02 mL 0.09 mL 0.19 mL

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产品说明书 for dTAG-47

分析证书/产品说明书
选择另一批次：
化学品安全技术说明书

参考文献 for dTAG-47

参考文献是支持产品生物活性的出版物。

Popay et al (2021) MYC regulates ribosome biogenesis and mitochondrial gene expression programs through its interaction with host cell factor-1. Elife 10 e60191 PMID: 33416496

Bryan et al (2020) WDR5 is a conserved regulator of protein synthesis gene expression. Nucleic Acids Res. 48 2924 PMID: 31996893

Sreekanth et al (2020) Chemogenetic system demonstrates that Cas9 longevity impacts genome editing outcomes. ACS Cent.Sci. 6 2228 PMID: 33376784

Brunetti et al (2018) Mutant NPM1 maintains the leukemic state through HOX expression. Cancer Cell. 34 499 PMID: 30205049

Weintraub et al (2017) YY1 is a structural regulator of enhancer-promoter loops. Cell 171 1573 PMID: 29224777

Layden et al (2021) A protocol for rapid degradation of endogenous transcription factors in mammalian cells and identification of direct regulatory targets. STAR Protoc. 2 100530 PMID: 34041503

If you know of a relevant reference for dTAG-47, please let us know.

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关键词: dTAG-47, dTAG-47 supplier, dTAG47, degraders, degrades, degradation, FKBP12F36V, fusion, protein, mutant, PROTAC, proteolysis, targeting, chimera, cereblon, PROTACs, TAG, Degradation, Platform, 7530, Tocris Bioscience

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该领域的文献

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

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TPD and Induced Proximity Research Product Guide

TPD and Induced Proximity Research Product Guide

This brochure highlights the tools and services available from Bio-Techne to support your Targeted Protein Degradation and Induced Proximity research, including:

  • Active Degraders
  • TAG Degradation Platform
  • Degrader Building Blocks
  • Assays for Protein Degradation
  • Induced Proximity Tools
Targeted Protein Degradation Poster

Targeted Protein Degradation Poster

Degraders (e.g. PROTACs) are bifunctional small molecules, that harness the Ubiquitin Proteasome System (UPS) to selectively degrade target proteins within cells. They consist of three covalently linked components: an E3 ubiquitin ligase ligand, a linker and a ligand for the target protein of interest. Authored in-house, this poster outlines the generation of a toolbox of building blocks for the development of Degraders. The characteristics and selection of each of these components are discussed. Presented at EFMC 2018, Ljubljana, Slovenia

Validating Targets for TPD Using dTAG Poster

Validating Targets for TPD Using dTAG Poster

The dTAG platform offers a generalizable strategy to degrade, in principle, any intracellular protein of interest (POI) and is a useful strategy for exploration and validation of targets. This poster presents a workflow solution for target validation, from custom TAG knock-in cell-lines for your POI, different dTAG Degraders to knockdown you POI, to automated assays for protein degradation using Simple WesternTM instruments. Data are also presented on the use of an antibody that recognizes the TAG domain (FKBP12F36V) for detection of degradation. Presented at TPD Europe, March 2022, London, UK.