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Filipin III

Cat. No. 6250 1 条评论 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： Fluorescent cholesterol stain, also polyene antibiotic. Used for: Fluorescent cholesterol stain. Binds sterol in fungal membranes. Application: confocal microscopy
化学名： (3R,4S,6S,8S,10R,12R,14R,16S,17E,19E,21E,23E,25E,27S,28R)-4,6,8,10,12,14,16,27-Octahydroxy-3-[(1R)-1-hydroxyhexyl]-17,28-dimethyloxacyclooctacosa-17,19,21,23,25-pentaen-2-one
纯度： ≥90% (HPLC)
说明书
引用文献
评论 (1)
文献 (1)
生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for Filipin III

Key information: filipin III is a fluorescent cholesterol stain; polyene antibiotic.

Used for: fluorescent cholesterol staining. Binds sterols in fungal membranes. Also inhibits formation of the pathological form of prion protein (PrP-res) from the normal membrane-bound (PrP-sen) form.

Application: confocal microscopy.

Properties and Photophysical Data: excitation and emission maxima (λ) are 405 nm and 420-480 nm, respectively.

技术数据 for Filipin III

分子量 654.83
公式 C35H58O11
储存 Store at -20°C
纯度 ≥90% (HPLC)
CAS Number 480-49-9
PubChem ID 6433553
InChI Key IMQSIXYSKPIGPD-ZPXVTHDFSA-N
Smiles C[C@@H](O1)[C@@H](O)/C=C/C=C/C=C/C=C/C=C(C)/[C@@H](O)C[C@H](O)C[C@H](O)C[C@H](O)C[C@H](O)C[C@H](O)C[C@H](O)[C@]([C@H](O)CCCCC)([H])C1=O

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Filipin III

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 3.27 5

制备储备液 for Filipin III

以下数据基于产品分子量 654.83。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
0.05 mM 30.54 mL 152.71 mL 305.42 mL
0.25 mM 6.11 mL 30.54 mL 61.08 mL
0.5 mM 3.05 mL 15.27 mL 30.54 mL
2.5 mM 0.61 mL 3.05 mL 6.11 mL

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产品说明书 for Filipin III

分析证书/产品说明书
选择另一批次：
化学品安全技术说明书

参考文献 for Filipin III

参考文献是支持产品生物活性的出版物。

Castanho et al (1992) Absorption and fluorescence spectra of polyene antibiotics in the presence of cholesterol. J.Biol.Chem. 267 204 PMID: 1730589

Marella et al (2002) Filipin prevents pathological prion protein accumulation by reducing endocytosis and inducing cellular PrP release. J.Biol.Chem. 277 25457 PMID: 11994310

Pipalia et al (2006) Automated microscopy screening for compounds that partially revert cholesterol accumulation in Niemann-Pick C cells. J.Lipid Res. 47 284 PMID: 16288097

If you know of a relevant reference for Filipin III, please let us know.

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关键词: Filipin III, Filipin III supplier, polyene, antibiotics, antifungals, prions, protein, inhibitors, inhibits, PrP, fluorescent, cholesterol, stain, Antibiotics, Prion, Protein, Labels, and, Stains, Fluorescent, Cholesterol, Probes, Lipid, Cell, Membrane, Antifungals, 6250, Tocris Bioscience

篇 Filipin III 的引用文献

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Filipin III 的评论

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Staining for intracellular cholesterol.
By Anonymous on 06/13/2019
分析类型： In Vitro
种属： Mouse
细胞系/组织： MEF

Fix the cells with 4% PFA for 15 min at room temperature; permeabilisation not required; incubate the cells with 0.5 mg/mL Fillipin in 3% BSA for 4 h at room temperature; mount the coverslips with DAPI-free moviol. Excite the cells with 405 nm laser, and collect signals at 410-500 nm.

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该领域的文献

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals