Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewFlutax 2
说明： Green-fluorescent taxol derivative; binds microtubules. Used for: imaging microtubules in live cells, isolated cytoskeletons and microtubule suspensions
化学名： (2aR,4S,4aS,6R,9S,11S,12S,12aR,12bS)-6,12b-Bis(acetyloxy)-9-[(2R,3S)-3-(benzoylamino)-2-hydroxy-1-oxo-3-phenylpropoxy]-12-(benzoyloxy)-2a,3,4,4a,5,6,9,10,11,12,12a,12b-dodecahydro-11-hydroxy-4a,8,13,13-tetramethyl-5-oxo-7,11-methano-1H-cyclodeca[3,4]benz[1,2-b]oxet-4-yl ester N-[(2',7'-difluoro-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)carbonyl]-L-alanine
纯度： ≥90% (HPLC)
生物活性 for Flutax 2
Key information: Flutax 2 is a green-fluorescent taxol derivative; for microtubules labeling in live cells.
Used for: imaging microtubules in live cells, isolated cytoskeletons and microtubule suspensions.
Application: fluorescence microscopy.
Properties and Photophysical Data: The fluorescent label on Flutax 2 is attached by derivatization of the 7-β-hydroxy group of native paclitaxel which permits selective binding of the probe to microtubules. Flutax 2 binds microtubules with high affinity (Ka ~ 107M-1). Flutax 2 binds to α, β tubulin dimers and stabilizes them. Flutax 2 is soluble and pH insensitive and is more photostable than Flutax 1 (Cat. No. 2226). Excitation and emission maxima (λ) are 496 nm and 526 nm, respectively.
Scientific Data
Fluorescence Imaging
View Larger
View Larger
Flutax 2 labeled HeLa cells. Flutax 2 was diluted in HBSS and applied to live HeLa cells at a concentration of 2 μM. HeLa cells were incubated for 1 hour at 37°C. After incubation was complete, Flutax 2 was washed off, and fresh HBSS was applied to the cells. Cells were not fixed because Flutax 2 staining is not retained at all after fixation (photobleach). Like Flutax 1, Flutax 2 staining in live cells diminishes very rapidly when exposed to light, so the cells should be exposed to light as little as possible to retain good signal.
Optical Data for Flutax 2
| λabs | 496 nm |
|---|---|
| λem | 526 nm |
| Extinction Coefficient (ε) | 80000 M-1cm-1 |
| Quantum Yield (φ) | 0.9 |
| Application | Fluorescence Microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
技术数据 for Flutax 2
| 分子量 | 1319.28 |
| 公式 | C71H64F2N2O21 |
| 储存 | Store at -20°C |
| 纯度 | ≥90% (HPLC) |
| CAS Number | 301844-13-3 |
| PubChem ID | 44571006 |
| InChI Key | UTOYCOKRJWZPNT-SQNCZLKBSA-N |
| Smiles | O=C1[C@@]2(C)C([C@@]3(OC(C)=O)[C@H](OC3)C[C@@H]2OC([C@H](C)NC(C4=CC=C(C5=C(C=C6F)C(OC7=C5C=C(F)C(O)=C7)=CC6=O)C(C(O)=O)=C4)=O)=O)[C@H](OC(C8=CC=CC=C8)=O)[C@@]9(O)C(C)(C)C([C@H]1OC(C)=O)=C(C)[C@@H](OC([C@H](O)[C@H](C%10=CC=CC=C%10)NC(C%11=CC=CC=C%11)=O)=O)C9 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for Flutax 2
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 1.32 | 1 |
制备储备液 for Flutax 2
以下数据基于产品分子量 1319.28。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.01 mM | 75.8 mL | 378.99 mL | 757.99 mL |
| 0.05 mM | 15.16 mL | 75.8 mL | 151.6 mL |
| 0.1 mM | 7.58 mL | 37.9 mL | 75.8 mL |
| 0.5 mM | 1.52 mL | 7.58 mL | 15.16 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
产品说明书 for Flutax 2
参考文献 for Flutax 2
参考文献是支持产品生物活性的出版物。
Lillo et al (2002) Location and properties of the Tax. binding center in microtubules: a picosecond laser study with fluorescent taxoids. Biochemistry. 41 12436 PMID: 12369834
Diaz et al (2000) Molecular recognition of Tax. by microtubules. Kinetics and thermodynamics of binding of fluorescent Tax. derivatives to an exposed site. J.Biol.Chem. 275 26265 PMID: 10818101
If you know of a relevant reference for Flutax 2, please let us know.
按标靶查看相关产品
关键词: Flutax 2, Flutax 2 supplier, paclitaxel, Flutax2, Taxol, fluorescent, dye, labeled, labelled, microtubules, tubulin, oregon, green, Microtubules, Mitosis, Organelle, and, Cell, Structure, Stains, Microtubule, Probes, 6254, Tocris Bioscience
篇 Flutax 2 的引用文献
引用文献是使用了 Tocris 产品的出版物。
目前没有 Flutax 2 的引用文献。 您是否知道使用了 Tocris Flutax 2 的优秀论文？ 请告知我们.
Flutax 2 的评论
目前没有该产品的评论。 Be the first to review Flutax 2 and earn rewards!
Have you used Flutax 2?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
该领域的文献
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。
Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals