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Submit ReviewForskolin
说明： Adenylyl cyclase activator
化学名： (3R,4aR,5S,6S,6aS,10S,10aR,10bS)-5-(Acetyloxy)-3-ethenyldodecahydro-6,10,10b-trihydroxy-3,4a,7,7,10a-pentamethyl-1H-naphtho[2,1-b]pyran-1-one
纯度： ≥98% (HPLC)
生物活性 for Forskolin
Forskolin, or coleonol, is a cell-permeable, potent, reversible and rapid activator of adenylyl cyclase, an enzyme that converts ATP to cAMP and pyrophosphate (EC50 = 0.5 μM). In combination with 5 other compounds, forskolin enables chemical reprogramming of mouse embryonic fibroblasts to pluripotent stem cells, without genetic factors: Valproic acid, sodium salt (Cat. No. 2815), RepSox (Cat. No. 3742), Tranylcypromine hydrochloride (Cat. No. 3852), CHIR 99021 (Cat. No. 4423) and 3-Deazaneplanocin A hydrochloride (Cat. No. 4703). In combination with IBMX (Cat. No. 2845), forskolin induces neural differentiation of mesenchymal stem cells by downregulating neuron restrictive silencer factor. In a rat model of Huntington's disease-like neurodegeneration, forskolin also exhibits neuroprotective effects. Hypotensive and vasodilatory agent.
Water-soluble analog, NKH 477 (Cat. No. 1603), and Forskolin synthesized to Ancillary Material Grade also available.
For more information about how Forskolin may be used, see our protocol: Transdifferentiating Fibroblasts into Neurons.
化合物库 for Forskolin
Forskolin is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. 了解 Tocris 化合物库的更多信息。
技术数据 for Forskolin
| 分子量 | 410.51 |
| 公式 | C22H34O7 |
| 储存 | Store at -20°C |
| 纯度 | ≥98% (HPLC) |
| CAS Number | 66575-29-9 |
| PubChem ID | 47936 |
| InChI Key | OHCQJHSOBUTRHG-KGGHGJDLSA-N |
| Smiles | C=C[C@](C3)(C)O[C@]2(C)[C@@H](OC(C)=O)[C@@H](O)[C@@]1([H])C(C)(C)CC[C@H](O)[C@@](C)1[C@](O)2C3=O |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for Forskolin
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| ethanol | 4.11 | 10 | |
| DMSO | 10.26 | 25 |
制备储备液 for Forskolin
以下数据基于产品分子量 410.51。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.25 mM | 9.74 mL | 48.72 mL | 97.44 mL |
| 1.25 mM | 1.95 mL | 9.74 mL | 19.49 mL |
| 2.5 mM | 0.97 mL | 4.87 mL | 9.74 mL |
| 12.5 mM | 0.19 mL | 0.97 mL | 1.95 mL |
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产品说明书 for Forskolin
参考文献 for Forskolin
参考文献是支持产品生物活性的出版物。
Awad et al (1983) Interactions of forskolin and adenylate cyclase. J.Biol.Chem. 258 2960 PMID: 6681815
Laurenza et al (1989) Forskolin: a specific stimulator of adenylyl cyclase or a diterpene with multiple sites of action? TiPS 10 442 PMID: 2692256
Seamon et al (1983) Structure-activity relationships for activation of adenylate cyclase by the diterpene forskolin and its derivatives. J.Med.Chem. 26 436 PMID: 6681845
Kim et al (2005) cAMP induces neuronal differentiation of mesenchymal stem cells via activation of extracellular signal-regulated kinase/MAPK. Neuroreport 16 1357 PMID: 16056139
Hou et al (2013) Pluripotent stem cells induced from mouse somatic cells by small-molecule compounds. Science 341 651 PMID: 23868920
Sato et al (2015) SnapShot: Growing Organoids from Stem Cells. Cell 161 1700 PMID: 26091044
Robbins et al (1996) Forskolin carbamates: binding and activation studies with type I adenylyl cyclase. J.Med.Chem. 39 2745 PMID: 8709105
If you know of a relevant reference for Forskolin, please let us know.
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关键词: Forskolin, Forskolin supplier, Adenylyl, cyclases, activator, Inositol, cAMP, Signaling, Signalling, adenylate, stem, cells, organoids, coleonol, Cyclase, Enzyme, Substrates, /, Activators, Neural, Stem, Cells, Organoids, 1099, Tocris Bioscience
86 篇 Forskolin 的引用文献
引用文献是使用了 Tocris 产品的出版物。 Forskolin 的部分引用包括：
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Forskolin 的评论
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Works well with organoids.
By
Gautam Kok on 11/19/2020
分析类型： In Vitro
种属： Human
细胞系/组织： Liver organoids
Culturing liver organoids - used to swell the organoids
Jurkat cells treated with Forskolin.
By
Tahmineh Safaie on 08/04/2020
分析类型： In Vitro
种属： Human
细胞系/组织： Jurkat
Jurkat cells were treated with different concentration of Forskolin for 15 minutes and the cAMP was measured (the cAMP levels are reversely correlated with Luminescent values.)
Validation.
By
Anonymous on 04/04/2020
分析类型： In Vitro
种属： Rat
细胞系/组织： Sprague-Dawley rats primary neuronal
10 μM for 30min
To start reprogramming, cultures were switched to reprogramming medium (ES medium supplemented with 10 mM Forskolin).
By
Anonymous on 12/04/2019
分析类型： In Vitro
种属： Mouse
细胞系/组织： ips
To start reprogramming, cultures were switched to reprogramming medium (ES medium supplemented with 10 mM Parnate, 3 mM Chir99021, 1 mM A83-01, and 10 mM Forskolin)
Forskolin increases intracellular cAMP levels.
By
Niroj Shrestha on 07/08/2019
分析类型： In Vitro
种属： Human
细胞系/组织： HEK293
HEK293 cells transfected with cAMP FRET sensor, Epac-S(H187) were perfused in normal tyrode solution and excited alternately at 430 nm and fluorescence was captured at 470 and 535 nm. Forskolin (10 µM) was added at 1 min to activate adenylyl cyclase and increase intracellular cAMP, which is detected as an increase in 470/535 fluorescence ratio.
a potent adenylyl cyclase.
By
Anonymous on 02/21/2018
种属： Rat
Forskolin activates adenylyl cyclase and increases intracellular cAMP. We used at 10 microM in cultured astrocytes to increase intracellular cAMP level.