主页 / Product Type / 荧光成像 / Fluorescent Probes and Stains / Enzyme Probes and Enzyme Substrates / GF-AFC

GF-AFC

Cat. No. 8143 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
Pricing Availability   Qty
说明： Cell permeable fluorogenic substrate cleaved by cytosolic aminopeptidases for non-lytic high throughput cell viability assays
化学名： (2S)-2-[(2-Aminoacetyl)amino]-N-[2-oxo-4-(trifluoromethyl)chromen-7-yl]-3-phenylpropanamide hydrochloride
纯度： ≥98% (HPLC)
说明书
引用文献
评论
文献 (1)
生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for GF-AFC

GF-AFC (glycyl-phenylalanyl-aminofluorocoumarin) is a cell permeable peptide fluorogenic substrate. GF-AFC is cleaved by cytosolic aminopeptidases for non-lytic high throughput cell viability assays. GF-AFC can be used as a substrate for measuring the activity of dipeptidyl peptidase 1 (DPP1), an intracellular lysosomal cysteinyl protease.

技术数据 for GF-AFC

分子量 469.85
公式 C21H18F3N3O4.HCl
储存 Store at -20°C
纯度 ≥98% (HPLC)
InChI Key GBCCOIYISILSJP-NTISSMGPSA-N
Smiles NCC(N[C@@H](CC1=CC=CC=C1)C(NC2=CC=C(C(O3)=C2)C(C(F)(F)F)=CC3=O)=O)=O.Cl

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for GF-AFC

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 46.98 100

制备储备液 for GF-AFC

以下数据基于产品分子量 469.85。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
1 mM 2.13 mL 10.64 mL 21.28 mL
5 mM 0.43 mL 2.13 mL 4.26 mL
10 mM 0.21 mL 1.06 mL 2.13 mL
50 mM 0.04 mL 0.21 mL 0.43 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

参考文献 for GF-AFC

参考文献是支持产品生物活性的出版物。

Liu et al (2017) Serum based fluorescent assay for evaluating dipeptidyl peptidase I activity in collagen induced arthritis rat model. Mol.Cell.Probes 32 5 PMID: 27771442

Niles et al (2007) A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers. Anal.Biochem. 366 197 PMID: 17512890

If you know of a relevant reference for GF-AFC, please let us know.

按标靶查看相关产品

关键词: GF-AFC, GF-AFC supplier, Cell, permeable, fluorogenic, substrate, cleaved, by, cytosolic, aminopeptidases, for, non-lytic, high, throughput, cell, viability, assays, cytotoxicity, live, cells, Enzyme, Probes, and, Substrates, 8143, Tocris Bioscience

篇 GF-AFC 的引用文献

引用文献是使用了 Tocris 产品的出版物。

目前没有 GF-AFC 的引用文献。 您是否知道使用了 Tocris GF-AFC 的优秀论文？ 请告知我们.

GF-AFC 的评论

目前没有该产品的评论。 Be the first to review GF-AFC and earn rewards!

Have you used GF-AFC?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

该领域的文献

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals