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Submit ReviewHoechst 33258
说明： Fluorescent dye for staining DNA
化学名： 4-[6-(4-Methyl-1-piperazinyl)[2,6'-bi-1H-benzimidazol]-2'-yl]phenol trihydrochloride
纯度： ≥98% (HPLC)
生物活性 for Hoechst 33258
Hoechst 33258 is a fluorescent dye. Binds the minor groove of DNA at A and T rich regions. Can be used as indicator of apoptosis.
Optical Data for Hoechst 33258
| λabs | 352 nm |
|---|---|
| λem | 461 nm |
| Application | Flow Cytometry |
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技术数据 for Hoechst 33258
| 分子量 | 533.88 |
| 公式 | C25H24N6O.3HCl |
| 储存 | Store at -20°C |
| 纯度 | ≥98% (HPLC) |
| CAS Number | 23491-45-4 |
| PubChem ID | 5360743 |
| InChI Key | OKQAASMPIOSOEX-UHFFFAOYSA-N |
| Smiles | CN(CC1)CCN1C2=CC(N=C(C3=CC(NC(C4=CC=C(O)C=C4)=N5)=C5C=C3)N6)=C6C=C2.Cl.Cl.Cl |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
产品说明书 for Hoechst 33258
参考文献 for Hoechst 33258
参考文献是支持产品生物活性的出版物。
Oberhammer et al (1993) Apoptotic death in epithelial cells: cleavage of DNA to 300 and/or 50 kb fragments prior to or in the absence of internucleosomal fragmentation. EMBO J. 12 3679 PMID: 8253089
Jin and Breslauer (1988) Characterization of the minor groove environment in a drug-DNA complex: bisbenzimide bound to the poly[d(AT)].poly[d(AT)]duplex. Proc.Natl.Acad.Sci.U.S.A. 85 8939 PMID: 2461559
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关键词: Hoechst 33258, Hoechst 33258 supplier, DNA, minor, groove, binds, fluorescent, dyes, probes, Fluorescent, Stains, Cell, Viability, and, Dyes, Counterstains, Flow, Cytometry, 5824, Tocris Bioscience
2 篇 Hoechst 33258 的引用文献
引用文献是使用了 Tocris 产品的出版物。 Hoechst 33258 的部分引用包括：
Terheyden-Keighley et al (2018) CXCR4/SDF1 signalling promotes sensory neuron clustering in vitro. Biol Open 7 PMID: 30135081
Moreno et al (2019) Mitotic replisome disassembly depends on TRAIP ubiquitin ligase activity. Life Sci Alliance 2 PMID: 30979826
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals