Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewHoechst Janelia Fluor® 526
说明： Fluorogenic, green-emitting DNA probe. Used for: fixed and live-cell imaging. Application: confocal microscopy, flow cytometry, super resolution microscopy (SRM)
别名： JF526-HST
化学名： 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-((2-(2-(2-(4-(4-(6-(4-methylpiperazin-1-yl)-1H,3'H-[2,5'-bibenzo[d]imidazol]-2'-yl)phenoxy)butanamido)ethoxy)ethoxy)ethyl)carbamoyl)benzoate tetratrifluoroacetate
纯度： ≥95% (HPLC)
生物活性 for Hoechst Janelia Fluor® 526
Key information: Hoechst Janelia Fluor® 526 is a fluorogenic, green-emitting DNA probe. Suitable for live cell imaging.
Used for: fixed and live-cell imaging.
Application: confocal microscopy, flow cytometry, super resolution microscopy (SRM).
Properties and Photophysical Data: preferentially stains and binds minor groove of AT-rich regions. When bound to A-T DNA, Hoechst Janelia Fluor® 526 exhibits a quantum yield (QY) of 0.126; when bound to G-C DNA Hoechst Janelia Fluor® 526 exhibits a quantum yield of 0.04. Fluorogenic, it fluoresces only once bound to DNA, enabling hassle-free no-wash experiments. Hoechst Janelia Fluor® 526 can be combined with fluorogenic red-emitting DNA probe Hoechst Janelia Fluor® 646 (Cat. No. 6804) for multiplexing experiments. Excitation and emission maxima (λ) are 531 nm and 549 nm, respectively; quantum yield = 0.126; extinction coefficient = 118,000 M-1cm-1.
To measure the absorbance spectrum of Hoechst Janelia Fluor® 526 we recommend the following solvent: TFE plus 0.1% TFA.
Scientific Data
Confocal Microscopy
View Larger
View Larger
Application of Hoechst Janelia Fluor® 526 in U2OS cells. Fixed U2OS cells were stained with Hoechst Janelia Fluor® 526 (2 µM) for 1 hour and imaged without an intermediate washing step on a LSM980 confocal microscope (Zeiss) using the following configuration: excitation: 514 nm / emission: 526–615 nm. Image kindly provided by Prof. Luke Lavis, Howard Hughes Medical Institute, Janelia Research Campus.
许可信息
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Hoechst Janelia Fluor® 526
| Emission Color | Yellow |
|---|---|
| λabs | 531 nm |
| λem | 549 nm |
| Extinction Coefficient (ε) | 118,000 M-1cm-1 |
| Quantum Yield (φ) | 0.126 |
| Application | Confocal Microscopy, Flow Cytometry, Super Resolution Microscopy (SRM) |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
技术数据 for Hoechst Janelia Fluor® 526
| 分子量 | 1641.27 |
| 公式 | C62H58F6N10O8.4CF3CO2H |
| 储存 | Store at -20°C |
| 纯度 | ≥95% (HPLC) |
| InChI Key | AFTKOWWBAAQRNW-UHFFFAOYSA-N |
| Smiles | CN1CCN(CC1)C2=CC=C3N=C(NC3=C2)C4=CC=C5N=C(NC5=C4)C6=CC=C(OCCCC(NCCOCCOCCNC(C7=CC(C8=C9C=C(F)/C(C=C9OC%10=C8C=C(F)C(N%11CC(C%11)(F)F)=C%10)=[N+]%12CC(C\%12)(F)F)=C(C=C7)C([O-])=O)=O)=O)C=C6.O=C(C(F)(F)F)O.O=C(C(F)(F)F)O.O=C(C(F)(F)F)O.O=C(C(F)(F)F)O |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for Hoechst Janelia Fluor® 526
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 12.99 | 10 |
制备储备液 for Hoechst Janelia Fluor® 526
以下数据基于产品分子量 1641.27。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 6.09 mL | 30.46 mL | 60.93 mL |
| 0.5 mM | 1.22 mL | 6.09 mL | 12.19 mL |
| 1 mM | 0.61 mL | 3.05 mL | 6.09 mL |
| 5 mM | 0.12 mL | 0.61 mL | 1.22 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
产品说明书 for Hoechst Janelia Fluor® 526
参考文献 for Hoechst Janelia Fluor® 526
参考文献是支持产品生物活性的出版物。
Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787
If you know of a relevant reference for Hoechst Janelia Fluor® 526, please let us know.
按标靶查看相关产品
关键词: Hoechst Janelia Fluor 526, Hoechst Janelia Fluor 526 supplier, Hoechst, Janelia, Fluor, 526, DNA, stains, stain, dyes, probes, fluorogenic, green-emitting, live, cell, imaging, JF526-HST, Fluorescent, Stains, Flow, Cytometry, Counterstains, 7313, Tocris Bioscience
篇 Hoechst Janelia Fluor® 526 的引用文献
引用文献是使用了 Tocris 产品的出版物。
目前没有 Hoechst Janelia Fluor® 526 的引用文献。 您是否知道使用了 Tocris Hoechst Janelia Fluor® 526 的优秀论文？ 请告知我们.
Hoechst Janelia Fluor® 526 的评论
目前没有该产品的评论。 Be the first to review Hoechst Janelia Fluor® 526 and earn rewards!
Have you used Hoechst Janelia Fluor® 526?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
该领域的文献
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。
Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals