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Submit ReviewJanelia Fluor® 526, NHS ester
说明： Fluorogenic yellow dye (Reactive group: NHS ester). Application: confocal microscopy, two-color lattice light-sheet microscopy, super resolution microscopy
别名： JF 526,NHS,JF526
化学名： 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate
生物活性 for Janelia Fluor® 526, NHS ester
Key Information: Janelia Fluor® 526, NHS ester is a fluorogenic yellow fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines.
Application: Suitable for confocal microscopy, two-color lattice light-sheet microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED. Cell permeable.
Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Can be multiplexed for two color imaging with Janelia Fluor® 635 NHS ester (Cat. No. 6419). Excitation and emission maxima (λ) are 526 nm and 550 nm, respectively; quantum yield = 0.87; extinction coefficient = 118,000 M-1cm-1; lactone-zwitterion equilibrium constant (KL-Z) = 0.005.
To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.
Please see the protocol for further information on protein/antibody labeling and conjugation.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
This product has been recently renamed. The previous name for this product was Janelia Fluor® 526, SE.
许可信息
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 526, NHS ester
| Emission Color | Yellow |
|---|---|
| Brightness | |
| λabs | 526 nm |
| λem | 550 nm |
| Extinction Coefficient (ε) | 118,000 M-1cm-1 |
| Quantum Yield (φ) | 0.87 |
| Closest Laser line | 488 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Application | Confocal Microscopy, Super Resolution Microscopy (SRM), Two-color Lattice Light-sheet Microscopy |
| Alternative For | Alexa Fluor® 532, Alexa Fluor® 514, Atto 532, CF514, CF532 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
技术数据 for Janelia Fluor® 526, NHS ester
| 分子量 | 659.5 |
| 公式 | C31H19F6N3O7 |
| 储存 | Store at -20°C |
| InChI Key | BCNDQOPFHGWJEL-UHFFFAOYSA-N |
| Smiles | O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC(F)=C(N4CC(F)(F)C4)C=C3O5)=C(C=C/6F)C5=CC6=[N+]7CC(F)(F)C\7)=C2 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for Janelia Fluor® 526, NHS ester
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 13.19 | 20 |
制备储备液 for Janelia Fluor® 526, NHS ester
以下数据基于产品分子量 659.5。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 7.58 mL | 37.91 mL | 75.82 mL |
| 1 mM | 1.52 mL | 7.58 mL | 15.16 mL |
| 2 mM | 0.76 mL | 3.79 mL | 7.58 mL |
| 10 mM | 0.15 mL | 0.76 mL | 1.52 mL |
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产品说明书 for Janelia Fluor® 526, NHS ester
参考文献 for Janelia Fluor® 526, NHS ester
参考文献是支持产品生物活性的出版物。
Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787
If you know of a relevant reference for Janelia Fluor® 526, NHS ester, please let us know.
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