Janelia Fluor^® 549, free acid

Cat. No. 6503 提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

Pricing	Availability		Qty

说明： Yellow dye (Reactive group: free acid). Application: flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging

化学名： 3,6-Di-1-azetidinyl-9-(2,5-dicarboxyphenyl)xanthylium, inner salt trifluoroacetate

纯度： ≥90% (HPLC)

文献 (1)

生物活性技术数据溶解性计算器说明书参考文献

生物活性 for Janelia Fluor^® 549, free acid

Key Information: Janelia Fluor^® 549, free acid is a yellow fluorescent dye; supplied with a free acid reactive group. Suitable for live cell imaging.

Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED. Used in protocol (2017 Grimm et al - see references below) for the synthesis of Janelia Fluor^® HaloTag^® and SNAP-tag^® ligands. Cell permeable.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 549 nm and 571 nm, respectively; quantum yield = 0.88; extinction coefficient = 101,000 M^-1cm^-1, A280 correction factor = 0.169; lactone-zwitterion equilibrium constant (K_L-Z) = 3.5.

Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

许可信息

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Janelia Fluor^® 549, free acid

Janelia Fluor<sup>®</sup> 549, free acid Dye Spectra

Emission Color	Yellow
Brightness
λ_abs	549 nm
λ_em	571 nm
Extinction Coefficient (ε)	101,000 M^-1cm^-1
Quantum Yield (φ)	0.88
Closest Laser line	532 nm
Reactive Group	Free acid
Cell Permeable	Yes
Correction Factor 280	0.169
Application	Confocal Microscopy, dSTORM, STED, Flow Cytometry, Live-cell Imaging
Alternative For	Alexa Fluor^® 546, Alexa Fluor^® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3

Alexa Fluor^® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

技术数据 for Janelia Fluor^® 549, free acid

分子量	568.5
公式	C₂₇H₂₂N₂O₅.C₂HF₃O₂
储存	Store at -20°C
纯度	≥90% (HPLC)
CAS Number	2245946-45-4
PubChem ID	137919862
InChI Key	GFUAWSMWTYTASE-UHFFFAOYSA-N
Smiles	OC(C1=CC=C(C(C(C2=CC=C(C=C2OC3=C/4)N5CCC5)=C3C=CC4=[N+]6CCC/6)=C1)C([O-])=O)=O.FC(F)(F)C(O)=O

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Janelia Fluor^® 549, free acid

	溶剂	最高浓度 mg/mL	最高浓度 mM
溶解性
溶解性	DMSO	45.45	100

制备储备液 for Janelia Fluor^® 549, free acid

以下数据基于产品分子量 568.5。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
1 mM	1.76 mL	8.8 mL	17.59 mL
5 mM	0.35 mL	1.76 mL	3.52 mL
10 mM	0.18 mL	0.88 mL	1.76 mL
50 mM	0.04 mL	0.18 mL	0.35 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt* g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1

Volume 1

Concentration 2

Volume 2

产品说明书 for Janelia Fluor^® 549, free acid

分析证书/产品说明书

化学品安全技术说明书

参考文献 for Janelia Fluor^® 549, free acid

参考文献是支持产品生物活性的出版物。

Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat. Methods 12 244 PMID: 25599551

Grimm et al (2017) Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments. Methods Mol.Biol. 1663 179 PMID: 28924668

Zheng et al Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787

If you know of a relevant reference for Janelia Fluor^® 549, free acid, please let us know.

按标靶查看相关产品

关键词: Janelia Fluor 549, free acid, Janelia Fluor 549, free acid supplier, JF549, Janelia, Fluor, 549,, Fluorescent, dye, free, acid, microscopy, cell, permeable, super, resolution, SE, STED, halo, tag, snap, flow, cytometry, Free, Acid, Dyes, Flow, Cytometry, 6503, Tocris Bioscience

篇 Janelia Fluor^® 549, free acid 的引用文献

引用文献是使用了 Tocris 产品的出版物。

目前没有 Janelia Fluor^® 549, free acid 的引用文献。您是否知道使用了 Tocris Janelia Fluor^® 549, free acid 的优秀论文？请告知我们.