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Submit ReviewJanelia Fluor® 549, Maleimide
说明： Yellow dye (Reactive group: maleimide). Application: flow cytometry, confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging
化学名： 2-(3-(Azetidin-1-ium-1-ylidene)-6-(azetidin-1-yl)-3H-xanthen-9-yl)-4-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)carbamoyl)benzoate trifluoroacetate
纯度： ≥95% (HPLC)
生物活性 for Janelia Fluor® 549, Maleimide
Key information: Janelia Fluor® 549, Maleimide is a yellow fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for live cell imaging.
Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED. Cell permeable.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 549 nm and 571 nm, respectively; quantum yield = 0.88; extinction coefficient = 101,000 M-1cm-1; A280 correction factor = 0.169; lactone-zwitterion equilibrium constant (KL-Z) = 3.5.
Please see the product protocol for further information and a guide to protein/antibody labeling.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
许可信息
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 549, Maleimide
| Emission Color | Yellow |
|---|---|
| Brightness | |
| λabs | 549 nm |
| λem | 571 nm |
| Extinction Coefficient (ε) | 101,000 M-1cm-1 |
| Quantum Yield (φ) | 0.88 |
| Closest Laser line | 532 nm |
| Reactive Group | Maleimide |
| Reactivity | Thiols |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.169 |
| Application | Confocal Imaging, dSTORM, STED, Flow Cytometry |
| Alternative For | Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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技术数据 for Janelia Fluor® 549, Maleimide
| 分子量 | 690.63 |
| 公式 | C33H28N4O6.C2HF3O2 |
| 储存 | Store at -20°C |
| 纯度 | ≥95% (HPLC) |
| InChI Key | UOOARLKDIKQOPO-UHFFFAOYSA-N |
| Smiles | O=C(NCCN1C(C=CC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CCC4)C=C3O5)=C(C=C/6)C5=CC6=[N+]7CCC\7)=C2.FC(C(O)=O)(F)F |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for Janelia Fluor® 549, Maleimide
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 69.06 | 100 |
制备储备液 for Janelia Fluor® 549, Maleimide
以下数据基于产品分子量 690.63。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.45 mL | 7.24 mL | 14.48 mL |
| 5 mM | 0.29 mL | 1.45 mL | 2.9 mL |
| 10 mM | 0.14 mL | 0.72 mL | 1.45 mL |
| 50 mM | 0.03 mL | 0.14 mL | 0.29 mL |
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产品说明书 for Janelia Fluor® 549, Maleimide
参考文献 for Janelia Fluor® 549, Maleimide
参考文献是支持产品生物活性的出版物。
Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy Nat.Methods. 12 244 PMID: 25599551
Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787
If you know of a relevant reference for Janelia Fluor® 549, Maleimide, please let us know.
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Fluorescent Dyes and Probes Research Product Guide
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