Janelia Fluor^® 585, NHS ester

Cat. No. 6418 提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

Pricing	Availability		Qty

说明： Orange dye (Reactive group: NHS ester). Application: confocal microscopy, super resolution microscopy, and two-photon excitation fluorescence microscopy. Suitable for live cell imaging

别名： JF 585,NHS

化学名： 1-[10-[2-Carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-7-(3,3-difluoro-1-azetidinyl)-9,9-dimethyl-2(9H)-anthracenylidene]-3,3-difluoroazetidinium, inner salt

纯度： ≥95% (HPLC)

实验方案 (1)

文献 (1)

生物活性技术数据溶解性计算器说明书参考文献

生物活性 for Janelia Fluor^® 585, NHS ester

Key Information: Janelia Fluor^® 585, NHS ester is an orange fluorogenic fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live cell imaging.

Applications: Suitable for confocal microscopy, two-photon excitation fluorescence microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). Cell permeable.

Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag^® and SNAP-tag^®. Excitation and emission maxima (λ) are 585 nm and 609 nm, respectively; quantum yield = 0.78; extinction coefficient = 156,000 M^-1cm^-1 (measured in Ethanol or TFE plus 0.1% TFA); A280 correction factor = 0.099; lactone-zwitterion equilibrium constant (K_L-Z) < 0.0001. Exhibits low non-specific background staining.

Please see the protocol for further information on protein/antibody labeling and conjugation.

To measure the absorbance spectrum of this dye we recommend the following solvent: trifluoroethanol plus 0.1% TFA.

We also offer Janelia Fluor^® conjugated antibodies and custom conjugation services.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.

This product has been recently renamed. The previous name for this product was Janelia Fluor^® 585, SE.

许可信息

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Janelia Fluor^® 585, NHS ester

Janelia Fluor<sup>®</sup> 585, NHS ester Dye Spectra

Emission Color	Orange
Brightness
λ_abs	585 nm
λ_em	609 nm
Extinction Coefficient (ε)	156,000 M^-1cm^-1
Quantum Yield (φ)	0.78
Closest Laser line	561 nm
Reactive Group	NHS ester
Reactivity	Primary amines
Cell Permeable	Yes
Correction Factor 280	0.099
Application	Confocal Microscopy, SRM, Two-photon Excitation Fluorescence Microscopy
Alternative For	Alexa Fluor^® 568, Atto Rho12, Atto Rho101, CF594

Alexa Fluor^® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

技术数据 for Janelia Fluor^® 585, NHS ester

分子量	649.6
公式	C₃₄H₂₇F₄N₃O₆
储存	Store at -20°C
纯度	≥95% (HPLC)
CAS Number	1811539-88-4
PubChem ID	132470913
InChI Key	GFNMIANIZWSVQR-UHFFFAOYSA-N
Smiles	O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CC(F)(F)C4)C=C3C5(C)C)=C(C=C/6)C5=CC6=[N+]7CC(F)(F)C\7)=C2

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Janelia Fluor^® 585, NHS ester

	溶剂	最高浓度 mg/mL	最高浓度 mM
溶解性
溶解性	DMSO	64.96	100

制备储备液 for Janelia Fluor^® 585, NHS ester

以下数据基于产品分子量 649.6。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
1 mM	1.54 mL	7.7 mL	15.39 mL
5 mM	0.31 mL	1.54 mL	3.08 mL
10 mM	0.15 mL	0.77 mL	1.54 mL
50 mM	0.03 mL	0.15 mL	0.31 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt* g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1

Volume 1

Concentration 2

Volume 2

产品说明书 for Janelia Fluor^® 585, NHS ester

分析证书/产品说明书

化学品安全技术说明书

参考文献 for Janelia Fluor^® 585, NHS ester

参考文献是支持产品生物活性的出版物。

Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 14 987 PMID: 28869757

Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787

If you know of a relevant reference for Janelia Fluor^® 585, NHS ester, please let us know.

按标靶查看相关产品

关键词: Janelia Fluor 585, NHS ester, Janelia Fluor 585, NHS ester supplier, JF585, Janelia, Fluor, 585-NHS, ester, fluorescent, dyes, NHS, self-labeling, tag, systems, microscopy, cell, permeable, super, resolution, JF, 585,, Amine, Reactive, Dyes, 6418, Tocris Bioscience

篇 Janelia Fluor^® 585, NHS ester 的引用文献

引用文献是使用了 Tocris 产品的出版物。

目前没有 Janelia Fluor^® 585, NHS ester 的引用文献。您是否知道使用了 Tocris Janelia Fluor^® 585, NHS ester 的优秀论文？请告知我们.