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Janelia Fluor® 669, Maleimide

Cat. No. 8097 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： Red dye (Reactive group: maleimide). Application: flow cytometry, confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging
化学名： 2-(3-(Azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((2-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)amino)-2-oxoethyl)thio)-3,5,6-trifluorobenzoate
纯度： ≥95% (HPLC)
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生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for Janelia Fluor® 669, Maleimide

Key information: Janelia Fluor® 669, Maleimide is a red fluorogenic fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for live cell imaging.

Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED. Can be multiplexed for two color imaging with Janelia Fluor® 549 SE (Cat. No. 6147). Cell permeable.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 669 nm and 689 nm, respectively.

Please see the product protocol for further information and a guide to protein/antibody labeling.

To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.

许可信息

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Janelia Fluor® 669, Maleimide

Janelia Fluor<sup>®</sup> 669, Maleimide Dye Spectra
Emission Color Red
λabs 669 nm
λem 689 nm
Reactive Group Maleimide
Reactivity Thiols
Cell Permeable Yes
Application Confocal Microscopy, dSTORM, STED, Flow Cytometry, Live-cell Imaging

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

技术数据 for Janelia Fluor® 669, Maleimide

分子量 718.82
公式 C36H33F3N4O5SSi
储存 Store at -20°C
纯度 ≥95% (HPLC)
InChI Key FUZVUVRRODXAGE-UHFFFAOYSA-N
Smiles C[Si](C1=CC(N2CCC2)=CC=C1C(C3=C(F)C(SCC(NCCN4C(C=CC4=O)=O)=O)=C(F)C(F)=C3C([O-])=O)=C5C=C/6)(C)C5=CC6=[N+]7CCC\7

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Janelia Fluor® 669, Maleimide

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 7.19 10

制备储备液 for Janelia Fluor® 669, Maleimide

以下数据基于产品分子量 718.82。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
0.1 mM 13.91 mL 69.56 mL 139.12 mL
0.5 mM 2.78 mL 13.91 mL 27.82 mL
1 mM 1.39 mL 6.96 mL 13.91 mL
5 mM 0.28 mL 1.39 mL 2.78 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

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产品说明书 for Janelia Fluor® 669, Maleimide

分析证书/产品说明书
化学品安全技术说明书

参考文献 for Janelia Fluor® 669, Maleimide

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