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Submit ReviewJFX™ 650, NHS ester 最新
说明： Deuterated Janelia Fluor® dye (Reactive group: NHS ester). Application: confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging
化学名： 2-(5,5-Dimethyl-3-(pyrrolidin-1-ium-1-ylidene-d8)-7-(pyrrolidin-1-yl-d8)-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate
纯度： ≥90% (HPLC)
生物活性 for JFX™ 650, NHS ester
Key Information: JFX™ 650, NHS ester is a red fluorescent deuterated Janelia Fluor® dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live cell imaging.
Application: Suitable for confocal microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Cell permeable.
Properties and Photophysical Data: JFX™ 650, NHS ester shows enhanced brightness, photostability and chromostability. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Excitation and emission maxima (λ) are 650 nm and 667 nm, respectively; quantum yield = 0.53; extinction coefficient = 17,600 M-1cm-1; lactone-zwitterion equilibrium constant (KL-Z) = 0.015.
Please see the protocol for further information on protein/antibody labeling and conjugation.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
JFX is a trademark owned by the Howard Hughes Medical Institute.
许可信息
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for JFX™ 650, NHS ester
| λabs | 650 nm |
|---|---|
| λem | 667 nm |
| Extinction Coefficient (ε) | 17,600 M-1cm-1 |
| Quantum Yield (φ) | 0.53 |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Application | Confocal Microscopy, dSTORM, STED, Flow Cytometry |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
技术数据 for JFX™ 650, NHS ester
| 分子量 | 637.86 |
| 公式 | C35H19D16N3O6Si |
| 储存 | Store at -20°C |
| 纯度 | ≥90% (HPLC) |
| CAS Number | 2396479-84-6 |
| PubChem ID | 153290020 |
| InChI Key | OJCIIHUZGDFMHK-UDNRTFQASA-N |
| Smiles | [2H]C(C([2H])([2H])C([2H])([2H])C/1([2H])[2H])([2H])[N+]1=C2C=CC3=C(C4=CC(C(ON5C(CCC5=O)=O)=O)=CC=C4C([O-])=O)C6=CC=C(N7C([2H])([2H])C([2H])([2H])C([2H])([2H])C7([2H])[2H])C=C6[Si](C)(C)C3=C/2 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
产品说明书 for JFX™ 650, NHS ester
参考文献 for JFX™ 650, NHS ester
参考文献是支持产品生物活性的出版物。
Grimm et al (2021) A general method to improve fluorophores using deuterated auxochromes. JACS 1 690 PMID: 34056637
If you know of a relevant reference for JFX™ 650, NHS ester, please let us know.
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