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Lactacystin

Cat. No. 2267 6 篇引用文献 1 条评论 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
Pricing Availability   Qty
说明： Cell-permeable, potent and selective proteasome inhibitor
化学名： (2R,3S,4R)-3-Hydroxy-2-[(1S)-1-hydroxy-2-methylpropyl]-4-methyl-5-oxo-2-pyrrolidinecarboxy-N-acetyl-L-cysteine thioester
纯度： ≥95% (HPLC)
说明书
引用文献 (6)
评论 (1)
文献 (2)
生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for Lactacystin

Lactacystin is a cell-permeable, potent and selective proteasome inhibitor. A Streptomyces metabolite that is thought to bind irreversibly to the active site N-terminal threonine residue of the catalytic β-subunit of the 20S proteasome, thereby inhibiting its chymotrypsin and trypsin-like activities. Induces neurite outgrowth in Neuro 2a neuroblastoma cells and has been reported to induce apoptosis in human monoblast U937 cells. Also inhibits NF-κB activation (IC50 = 10 μM).

技术数据 for Lactacystin

分子量 376.42
公式 C15H24N2O7S
储存 Store at -20°C
纯度 ≥95% (HPLC)
CAS Number 133343-34-7
PubChem ID 6610292
InChI Key DAQAKHDKYAWHCG-RWTHQLGUSA-N
Smiles O=C1N[C@]([C@@](SC[C@H](NC(C)=O)[C@](O)=O)=O)([C@H](C(C)C)O)[C@@H](O)[C@H]1C

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Lactacystin

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
water 3.76 10

制备储备液 for Lactacystin

以下数据基于产品分子量 376.42。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
0.1 mM 26.57 mL 132.83 mL 265.66 mL
0.5 mM 5.31 mL 26.57 mL 53.13 mL
1 mM 2.66 mL 13.28 mL 26.57 mL
5 mM 0.53 mL 2.66 mL 5.31 mL

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产品说明书 for Lactacystin

分析证书/产品说明书
选择另一批次：
化学品安全技术说明书

参考文献 for Lactacystin

参考文献是支持产品生物活性的出版物。

Omura et al (1991) Lactacystin, a novel microbial metabolite, induces neuritogenesis of neuroblastoma cells. J.Antibiot. 44 113 PMID: 1848215

Fenteany et al (1995) Inhibition of proteasome activities and subunit-specific amino-terminal threonine modification by lactacystin. Science 268 726 PMID: 7732382

Bellas et al (1997) Inhibition of NF-κB activity induces apoptosis in murine hepatocytes. Am.J.Pathol. 151 891 PMID: 9327720

If you know of a relevant reference for Lactacystin, please let us know.

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关键词: Lactacystin, Lactacystin supplier, Cell-permeable, potent, selective, proteasome, inhibitors, inhibits, Proteasome, Proteinases, Proteases, 2267, Tocris Bioscience

6 篇 Lactacystin 的引用文献

引用文献是使用了 Tocris 产品的出版物。 Lactacystin 的部分引用包括：

Saldate et al (2018) The ubiquitin-proteasome system functionally links neuronal Tomosyn-1 to dendritic morphology. J Biol Chem 293 2232 PMID: 29269412

Pandya et al (2014) Glucocorticoid regulates TrkB protein levels via c-Cbl dependent ubiquitination: a decrease in c-Cbl mRNA in the prefrontal cortex of suicide subjects. Psychoneuroendocrinology 45 108 PMID: 24845182

Wen et al (2015) FBG1 Is the Final Arbitrator of A1AT-Z Degradation. PLoS One 10 e0135591 PMID: 26295339

Warchal et al (2019) Evaluation of Machine Learning Classifiers to Predict Compound Mechanism of Action When Transferred across Distinct Cell Lines. SLAS Discov 24 224 PMID: 30694704

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Lactacystin 的评论

平均评分: 4 (Based on 1 Review.)

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lactacystin (10 M, 4 h).
By Anonymous on 12/23/2019
分析类型： In Vitro
种属： Rat
细胞系/组织： rat hippocampal neuronal

To tested the effects of inhibiting the proteolytic activity of the 26S proteasome complex via bath application of MG132 (50 M, 4 h) or lactacystin (10 M, 4 h) versus DMSO vehicle control.

PMID: 29269412
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