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Submit ReviewPolyamine Supplement x1000 (lyophilized)
说明： Media supplement to boost cell growth; used as component of CEPT cocktail to enhance stem cell viability
生物活性
Polyamine Supplement x1000 (lyophilized) is a media supplement comprising a lyophilized mixture of Putrescine, Spermine (Cat. No. 0958) and Spermidine. The product is used as a component of the CEPT cocktail, in combination with Emricasan (Cat. No. 7310), Chroman 1 (Cat. No. 7163) and trans-ISRIB (Cat. No. 5284) to enhance viability of human pluripotent stem cells (hPSCs) and their differentiated progeny. Polyamine Supplement x1000 (lyophilized) also improves survival of pluripotent and differentiated cells during cryopreservation, organoid formation, single-cell cloning, and genome editing.
NOTE: This reagent is provided lyophilized. To use prepare a 1000x stock solution by reconstituting vial contents in 5 mL of ddH2O and filter sterilize before use. Add 1 mL of sterile resuspended polyamines supplement (1000x stock) per 1 L of media.
技术数据
| 储存 | Store at -20°C |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
产品说明书
参考文献
参考文献是支持产品生物活性的出版物。
Chen et al (2021) A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells. Nat.Methods 18 528 PMID: 33941937
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该领域的文献
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。
Stem Cell Research Product Guide
This product guide provides a background to the use of small molecules in stem cell research and lists over 200 products for use in:
- Self-renewal and Maintenance
- Differentiation
- Reprogramming
- Organoid Generation
- GMP and Ancillary Material Grade Products
Stem Cells Scientific Review
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.
Stem Cell Workflow Poster
Stem cells have potential as a source of cells and tissues for research and treatment of disease. This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. Advantages of using small molecules are also highlighted.
Stem Cells Poster
Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids.