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Submit ReviewChroman 1
Description: Highly potent and selective ROCK 2 inhibitor; improves cell survival after cryogenesis
Chemical Name: (3S)-N-[2-[2-(Dimethylamino)ethoxy]-4-(1H-pyrazol-4-yl)phenyl]-3,4-dihydro-6-methoxy-2H-1-benzopyran-3-carboxamide dihydrochloride
Purity: ≥98% (HPLC)
Biological Activity for Chroman 1
Chroman 1 is a highly potent and selective ROCK2 (Rho-kinase) inhibitor (IC50 values are 1 and 52 pM at ROCK2 and ROCK1, respectively). Chroman 1 exhibits >2000-fold selectivity for ROCK2 over a range of related kinases including MRCK, PKA, and AKT1 (IC50 values are 150, >20000, and >20000 nM, respectively). Chroman 1 promotes survival of pluripotent stem cells (PSCs) in culture when used in combination with Emricasan (Cat. No. 7310), Polyamine Supplement x1000 (lyophilized) (Cat. No. 7739), and trans-ISRIB (Cat. No. 5284), a combination known as CEPT. When used as part of the CEPT cocktail, Chroman 1 also improves survival of differentiated cells following cryopreservation with cardiomyocyte cell survival increasing by 36% and motor neuron survival improving by 63% compared with DMSO controls.
Compound Libraries for Chroman 1
Chroman 1 is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Kinase Inhibitor Library and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.
Technical Data for Chroman 1
| M. Wt | 509.43 |
| Formula | C24H28N4O4.2HCl |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| InChI Key | ROFMCPHQNWGXGE-SFHVURJKSA-N |
| Smiles | COC1=CC=C2OC[C@@H](C(NC3=C(C=C(C4=CNN=C4)C=C3)OCCN(C)C)=O)CC2=C1.Cl.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Chroman 1
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 50.94 | 100 | |
| water | 2.55 | 5 |
Preparing Stock Solutions for Chroman 1
The following data is based on the product molecular weight 509.43. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.96 mL | 9.81 mL | 19.63 mL |
| 5 mM | 0.39 mL | 1.96 mL | 3.93 mL |
| 10 mM | 0.2 mL | 0.98 mL | 1.96 mL |
| 50 mM | 0.04 mL | 0.2 mL | 0.39 mL |
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Product Datasheets for Chroman 1
References for Chroman 1
References are publications that support the biological activity of the product.
Chen et al (2011) Asymmetric synthesis of potent chroman-based Rho kinase (ROCK-II) inhibitors. Med.Chem.Commun. 2 73
Chen et al (2021) A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells. Nat.Methods 18 528 PMID: 33941937
If you know of a relevant reference for Chroman 1, please let us know.
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Keywords: Chroman 1, Chroman 1 supplier, Selective, p160, ROCK, inhibitors, inhibits, Rho-Kinase, Rho-Associated, Coiled-Coil, Kinases, ROK, stem, cells, organoids, induced, pluripotent, ROCKI, ROCK1, ROCKII, ROCK2, reprogramming, cryopreservation, differentiation, cell, freezing, media, cryogenesis, self-renewal, th, Rho-kinases, Stem, Cell, Proliferation, Reprogramming, Organoids, Neural, Cells, CEPT, Cocktail, 7163, Tocris Bioscience
2 Citations for Chroman 1
Citations are publications that use Tocris products. Selected citations for Chroman 1 include:
Fujimoto et al (2024) SARS-CoV-2-induced disruption of a vascular bed in a microphysiological system caused by type-I interferon from bronchial organoids. Lab.Chip. PMID: 38252025
Windham et al (2024) APOE traffics to astrocyte lipid droplets and modulates triglyceride saturation and droplet size. J.Cell.Biol. 223 e202305003 PMID: 38334983
Do you know of a great paper that uses Chroman 1 from Tocris? Please let us know.
Reviews for Chroman 1
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Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Stem Cells Scientific Review
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.
Stem Cell Workflow Poster
Stem cells have potential as a source of cells and tissues for research and treatment of disease. This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. Advantages of using small molecules are also highlighted.
Stem Cells Poster
Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids.