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Submit ReviewPA Janelia Fluor® 549, NHS ester
说明： Yellow photoactivatable dye (Reactive group: NHS ester). Application: super resolution microscopy (SRM), single molecule tracking and live cell imaging
化学名： 2,5-Dioxo-1-pyrrolidinyl 3',6'-di-1-azetidinyl-2-diazo-2,3-dihydro-3-oxospiro[1H-indene-1,9'-[9H]xanthene]-6-carboxylate
纯度： ≥90% (HPLC)
生物活性 for PA Janelia Fluor® 549, NHS ester
Key information: PA Janelia Fluor® 549, NHS ester is a yellow photoactivatable fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live and fixed cell imaging.
Application: Suitable for single molecule tracking and super resolution microscopy (SRM) in live cells, specifically sptPALM (live cells) and PALM (fixed cells) techniques.
Properties and Photophysical Data: Non-fluorescent until activated at 405 nm. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Can be multiplexed with PA Janelia Fluor® 646, NHS ester (Cat. No. 6150) to perform two-color sptPALM in live cells with activation at 405 nm. Photochemical quantum yield of uncaging = 2.2%, with improved yield upon protein conjugation. Excitation and emission maxima (λ) are 551 - 553 nm and 570 - 573 nm, respectively.
Please see the protocol for further information on protein/antibody labeling and conjugation.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
This product has been recently renamed. The previous name for this product was PA Janelia Fluor® 549, SE.
许可信息
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
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技术数据 for PA Janelia Fluor® 549, NHS ester
| 分子量 | 575.58 |
| 公式 | C32H25N5O6 |
| 储存 | Store at -20°C |
| 纯度 | ≥90% (HPLC) |
| CAS Number | 1811539-42-0 |
| PubChem ID | 126569714 |
| InChI Key | ZJZWRNGNJDEPEQ-UHFFFAOYSA-N |
| Smiles | O=C(ON1C(CCC1=O)=O)C2=CC=C(C(C3=[N+]=[N-])=O)C(C43C5=CC=C(N6CCC6)C=C5OC7=CC(N8CCC8)=CC=C47)=C2 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for PA Janelia Fluor® 549, NHS ester
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 5.76 | 10 |
制备储备液 for PA Janelia Fluor® 549, NHS ester
以下数据基于产品分子量 575.58。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 17.37 mL | 86.87 mL | 173.74 mL |
| 0.5 mM | 3.47 mL | 17.37 mL | 34.75 mL |
| 1 mM | 1.74 mL | 8.69 mL | 17.37 mL |
| 5 mM | 0.35 mL | 1.74 mL | 3.47 mL |
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产品说明书 for PA Janelia Fluor® 549, NHS ester
参考文献 for PA Janelia Fluor® 549, NHS ester
参考文献是支持产品生物活性的出版物。
Grimm et al (2016) Bright photoactivatable fluorophores for single-molecule imaging. Nat.Methods 13 985 PMID: 27776112
Li et al (2016) Real-time imaging of Huntingtin aggregates diverting target search and gene transcription. eLife 5 e17056 PMID: 27484239
Hong et al (2009) Phosphorylation of the RNA polymerase II C-terminal domain by TFIIH kinase is not essential for transcription of Saccharomyces cerevisiae genome. Proc.Natl.Acad.Sci.USA. 106 14276 PMID: 19666497
If you know of a relevant reference for PA Janelia Fluor® 549, NHS ester, please let us know.
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关键词: PA Janelia Fluor 549, NHS ester, PA Janelia Fluor 549, NHS ester supplier, PA, JF549, Photoactivatable, caged, Janelia, Fluor, 549-NHS, ester, fluorescent, dyes, NHS, self-labeling, tag, systems, microscopy, cell, permeable, super, resolution, 549-SE, Dyes, 6149, Tocris Bioscience
1 篇 PA Janelia Fluor® 549, NHS ester 的引用文献
引用文献是使用了 Tocris 产品的出版物。 PA Janelia Fluor® 549, NHS ester 的部分引用包括：
Genshaft et al (2021) Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes Nat Commun 12 4995 PMID: 34404785
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