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PA Janelia Fluor® 646, Maleimide

Cat. No. 8133 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： Red photoactivatable dye (Reactive group: maleimide). Application: flow cytometry, confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging
化学名： 3,7-Di(azetidin-1-yl)-2'-diazo-N-(2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)-5,5-dimethyl-3'-oxo-2',3'-dihydro-5H-spiro[dibenzo[b,e]siline-10,1'-indene]-6'-carboxamide
纯度： ≥90% (HPLC)
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生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for PA Janelia Fluor® 646, Maleimide

Key information: PA Janelia Fluor® 646, Maleimide is a red cell-permeable photoactivatable fluorescent dye; supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for live and fixed cell imaging.

Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 649 - 651 nm and 663 - 665 nm, respectively.

Please see the product protocol for further information and a guide to protein/antibody labeling.

Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.

许可信息

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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技术数据 for PA Janelia Fluor® 646, Maleimide

分子量 642.79
公式 C36H34N6O4Si
储存 Store at -20°C
纯度 ≥90% (HPLC)
InChI Key AUOMWYIWQORWPM-UHFFFAOYSA-N
Smiles O=C(C1=CC=C(C(C23C4=CC=C(C=C4[Si](C)(C5=CC(N6CCC6)=CC=C35)C)N7CCC7)=C1)C(C2=[N+]=[N-])=O)NCCN8C(C=CC8=O)=O

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for PA Janelia Fluor® 646, Maleimide

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 6.43 10

制备储备液 for PA Janelia Fluor® 646, Maleimide

以下数据基于产品分子量 642.79。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
0.1 mM 15.56 mL 77.79 mL 155.57 mL
0.5 mM 3.11 mL 15.56 mL 31.11 mL
1 mM 1.56 mL 7.78 mL 15.56 mL
5 mM 0.31 mL 1.56 mL 3.11 mL

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产品说明书 for PA Janelia Fluor® 646, Maleimide

分析证书/产品说明书
化学品安全技术说明书

参考文献 for PA Janelia Fluor® 646, Maleimide

参考文献是支持产品生物活性的出版物。

Grimm et al (2016) Bright photoactivatable fluorophores for single-molecule imaging. Nat.Methods 13 985 PMID: 27776112

If you know of a relevant reference for PA Janelia Fluor® 646, Maleimide, please let us know.

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关键词: PA Janelia Fluor® 646, Maleimide, PA Janelia Fluor® 646, Maleimide supplier, PA, JF646, Photoactivatable, caged, Janelia, Fluor, 646-Maleimide, fluorescent, dyes, microscopy, cell, permeable, super, resolution, SRM, Dyes, Sulfhydryl, Reactive, 8133, Tocris Bioscience

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