Phalloidin-Janelia Fluor^® 646

Cat. No. 7201 提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

Pricing	Availability		Qty

说明： Red-fluorescent F-actin probe. Binds and labels F-actin. Used for: F-actin labelling. Application: confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED, flow cytometry

别名： Phalloidin-JF646

纯度： ≥90% (HPLC)

文献 (1)

生物活性 Scientific Data 技术数据说明书参考文献

生物活性 for Phalloidin-Janelia Fluor^® 646

Key information: Phalloidin-Janelia Fluor^®646 is a red fluorescent F-actin probe. It binds and labels specifically F-actin.

Used for: Phalloidin-Janelia Fluor^® 646 can be used to visualize and quantify F-actin in tissue sections, cell cultures or cell-free experiments with optimal results in formaldehyde-fixed and permeabilized cells.

Application: confocal microscopy, super-resolution microscopy (SRM) including dSTORM and STED and flow cytometry.

Properties and Photophysical Data: Phalloidin-Janelia Fluor^® 646 is composed of the F-actin probe, Phalloidin (Cat. No. 4535), conjugated to Janelia Fluor^® 646 (Cat. No. 6148). Excitation and emission maxima (λ) are 646 nm and 664 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M^-1cm^-1.

Scientific Data

Confocal Microscopy

View Larger

Application of Phalloidin-Janelia Fluor^® 646. HeLa cells were fixed for 10 minutes with 4% formaldehyde in PBS and permeabilized with PBS/0.25% Triton X-100 for 6 minutes. An additional post-fixation step was performed twice with 1% formaldehyde in PBS followed by 3 washes with PBS. Phalloidin Janelia Fluor^® 646 was prepared as a 10 μM stock solution diluted in methanol and diluted to a working concentration of 20 nM in PBS. Stained cells were imaged with a Leica SP8X WLL upright confocal microscope. Image kindly provided by Brigitte Bergner, Steffen Dietzel, Core Facility Bioimaging at the Biomedical Center of the Ludwig-Maximilians-Universität München.

Confocal Microscopy

View Larger

许可信息

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Phalloidin-Janelia Fluor^® 646

Phalloidin-Janelia Fluor<sup>®</sup> 646 Dye Spectra

Emission Color	Red
Brightness
λ_abs	646 nm
λ_em	664 nm
Extinction Coefficient (ε)	152,000 M^-1cm^-1
Quantum Yield (φ)	0.54
Closest Laser line	640 nm
Application	Confocal Imaging, dSTORM, STED, Flow Cytometry

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

技术数据 for Phalloidin-Janelia Fluor^® 646

分子量	1266.51
公式	C₆₄H₇₅N₁₁O₁₃SSi
储存	Store at -20°C
纯度	≥90% (HPLC)
InChI Key	AOXMEZGPCIYGOB-BNAQDRCBSA-N
Smiles	O=C([C@H](NC([C@H](C)NC([C@H](C[C@](O)(C)CNC(C1=CC=C(C([O-])=O)C(C2=C(C=C/3)C([Si](C)(C)C4=CC(N5CCC5)=CC=C42)=CC3=[N+]6CCC\6)=C1)=O)N7)=O)=O)[C@H](C)O)N[C@H]8CSC(N9)=C(C[C@H](NC([C@H](C)NC([C@@H]%10C[C@@H](O)CN%10C8=O)=O)=O)C7=O)C%11=C9C=CC=C%11

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

产品说明书 for Phalloidin-Janelia Fluor^® 646

分析证书/产品说明书

化学品安全技术说明书

参考文献 for Phalloidin-Janelia Fluor^® 646

参考文献是支持产品生物活性的出版物。

Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods 12 244 PMID: 25599551

Cooper et al (1987) Effects of cytochalasin and phalloidin on actin. J.Cell.Biol. 105 1473 PMID: 3312229

If you know of a relevant reference for Phalloidin-Janelia Fluor^® 646, please let us know.

按标靶查看相关产品

关键词: Phalloidin-Janelia Fluor 646, Phalloidin-Janelia Fluor 646 supplier, Phalloidin, Janelia, Fluor, 646, red, fluorescent, F-actin, probes, dyes, stains, cytoskeletal, cytoskeleton, Phalloidin-JF646, Fluorescent, Actin, Probes, 7201, Tocris Bioscience

篇 Phalloidin-Janelia Fluor^® 646 的引用文献

引用文献是使用了 Tocris 产品的出版物。

目前没有 Phalloidin-Janelia Fluor^® 646 的引用文献。您是否知道使用了 Tocris Phalloidin-Janelia Fluor^® 646 的优秀论文？请告知我们.