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Submit ReviewKey information: Phalloidin-TRITC is a red-orange fluorescent cytoskeleton stain. It binds and labels specifically F-actin.
Used for: Phalloidin-TRITC can be used to visualize and quantify F-actin in tissue sections, cell cultures or cell-free experiments with optimal results in formaldehyde-fixed and permeabilized cells.
Application: fluorescent microscopy, immunofluorescence (IHC, ICC).
Properties and Photophysical Data: Phalloidin-TRITC is composed of the F-actin probe, Phalloidin (Cat. No. 4535), conjugated to Tetramethylrhodamine isothiocyanate (TRITC) dye. Phalloidin binds F-actin with high selectivity allowing Phalloidin-TRITC to stain actin with high-contrast discrimination. Excitation and emission maxima (λ) are 540 nm and 565 nm, respectively.
We suggest making up a stock solution of this product by dissolving the vial contents in 1.5 mL methanol or DMSO. Please refer to the protocol for further information on using this product.
Applications in HeLa Cells for Phalloidin-TRITC. Application of Phalloidin-TRITC: HeLa cells were labeled with 150 nM Phalloidin-TRITC for 20 minutes. Counterstained with DAPI (Catalog # 5748).
分子量 | 1231.4 |
公式 | C60H70N12O13S2 |
储存 | Store at -20°C |
纯度 | ≥90% (HPLC) |
CAS Number | 915013-10-4 |
Smiles | C[C@H](O)[C@H]1NC(=O)C(C)NC(=O)[C@H](C[C@@](C)(O)CNC(=S)NC2=CC(C([O-])=O)=C(C=C2)C2=C3C=CC(C=C3OC3=C2C=CC(=C3)N(C)C)=[N+](C)C)NC(=O)[C@@H]2CC3=C(NC4=C3C=CC=C4)SC[C@@H](NC1=O)C(=O)N1C[C@H](O)C[C@H]1C(=O)N[C@@H](C)C(=O)N2 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
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关键词: Phalloidin-TRITC, Phalloidin-TRITC supplier, red, orange, fluorescent, cytoskeleton, actin, filament, stain, probe, Organelle, and, Cell, Structure, Stains, Fluorescent, Actin, Probes, 5783, Tocris Bioscience
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