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Submit ReviewPMSF
说明： Non-selective protease inhibitor; also blocks anandamide metabolism
化学名： Phenylmethylsulfonyl fluoride
纯度： ≥98%
生物活性 for PMSF
PMSF is a non-selective protease inhibitor; irreversibly sulfonates key active site serine residues in serine proteases, acetylcholinesterases, and thiol proteases. Blocks metabolism of anandamide (Cat. No. 1339) in a rodent model of cannabimimetic activity. Unstable in water (t1/2 ~ 60 mins at pH 7.5). Brain penetrant.
技术数据 for PMSF
| 分子量 | 174.19 |
| 公式 | C7H7FO2S |
| 储存 | Desiccate at RT |
| 纯度 | ≥98% |
| CAS Number | 329-98-6 |
| PubChem ID | 4784 |
| InChI Key | YBYRMVIVWMBXKQ-UHFFFAOYSA-N |
| Smiles | O=S(CC1=CC=CC=C1)(F)=O |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for PMSF
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| ethanol | 17.42 | 100 | |
| DMSO | 17.42 | 100 |
制备储备液 for PMSF
以下数据基于产品分子量 174.19。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 5.74 mL | 28.7 mL | 57.41 mL |
| 5 mM | 1.15 mL | 5.74 mL | 11.48 mL |
| 10 mM | 0.57 mL | 2.87 mL | 5.74 mL |
| 50 mM | 0.11 mL | 0.57 mL | 1.15 mL |
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产品说明书 for PMSF
参考文献 for PMSF
参考文献是支持产品生物活性的出版物。
Turini et al (1969) The action of phenylmethylsulfonyl fluoride on human acetylcholinesterase, chymotrypsin and trypsin. J.Pharmacol.Exp.Ther. 167 98 PMID: 4306345
Compton & Martin (1997) The effect of the enzyme inhibitor phenylmethylsulfonyl fluoride on the pharmacological effect of anandamide in the mouse model of cannabimimetic activity. J.Pharmacol.Exp.Ther. 283 1138 PMID: 9399986
Day et al (2001) Role of fatty acid amide hydrolase in the transport of the endogenous cannabinoid anandamide. Mol.Pharmacol. 59 1369 PMID: 11353795
If you know of a relevant reference for PMSF, please let us know.
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关键词: PMSF, PMSF supplier, proteases, inhibits, inhibitors, irreversible, sulfonation, brain, penetrant, FAAH, anandamide, fatty, acid, amide, hydrolase, Other, Proteases, 4486, Tocris Bioscience
3 篇 PMSF 的引用文献
引用文献是使用了 Tocris 产品的出版物。 PMSF 的部分引用包括：
Partha et al (2023) Structural basis of selective cannabinoid CB2 receptor activation. Nat Commun 14 1447 PMID: 36922494
Yan et al (2021) Rapid assessment of DCLK1 inhibitors using a peptide substrate mobility shift assay. STAR Protoc 2 100587 PMID: 34159321
Yingqiong et al (2021) A simple protocol to produce mature human-induced pluripotent stem cell-derived cardiomyocytes. STAR Protoc 2 100912 PMID: 34755117
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PMSF 的评论
平均评分: 4 (Based on 1 Review.)
Have you used PMSF?
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Works well for protein isolation.
By
Nicole Brown on 01/07/2019
分析类型： In Vivo
种属： Mouse
细胞系/组织： Kidney
Used to isolate protein from kidney tissue from wild type or IL-33 deficient mice that underwent cisplatin-induced kidney injury; several proteins were detected by immunoblot analysis; even after several freeze thaws, data has been consistent*additionally added a protease inhibitor cocktail and phosphatase inhibitor cocktail + RIPA for protein isolation
Does not contain phosphatase inhibitors; add these to protease inhibitor mix if analyzing phosphorylated proteins
